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Mouse Anti-MAP1LC3A Recombinant Antibody (4C8-B7-B9) (CBMAB-L2577-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3A). The antibody can be used for immunoassay techniques, such as WB.
See all MAP1LC3A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4C8-B7-B9
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Recombinant protein of human LC3A.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Microtubule Associated Protein 1 Light Chain 3 Alpha
Introduction
MAP1LC3A is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes).Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Entrez Gene ID
84557
UniProt ID
Q9H492
Alternative Names
Microtubule Associated Protein 1 Light Chain 3 Alpha; Autophagy-Related Ubiquitin-Like Modifier LC3 A; MAP1 Light Chain 3-Like Protein 1; MAP1A/MAP1B Light Chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-Associated Proteins 1A/1B Light Chain 3A; Microtubule-Associated Protein 1 Light Chain 3 Alpha; Microtubule-Associated Proteins 1A/1B Light Chain 3
Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141).
While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).
Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537).
Biological Process
Autophagosome assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Autophagosome maturationManual Assertion Based On ExperimentIDA:UniProtKB
Autophagy of mitochondrionManual Assertion Based On ExperimentIGI:MGI
Cellular response to amino acid starvationIEA:Ensembl
Cellular response to copper ionIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Cellular response to nitrogen starvationManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to starvationManual Assertion Based On ExperimentIDA:UniProtKB
MacroautophagyManual Assertion Based On ExperimentIDA:UniProtKB
Response to iron(II) ionIEA:Ensembl
Response to lead ionIEA:Ensembl
Response to morphineIEA:Ensembl
Cellular Location
Cytoplasmic vesicle, autophagosome membrane
Endomembrane system
Cytoplasm, cytoskeleton
LC3-II binds to the autophagic membranes.
PTM
The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:15187094, PubMed:30661429).
The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989).
ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:32686895, PubMed:33909989).
In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989).
ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429, PubMed:33909989).
ATG4D is the main enzyme for delipidation activity (By similarity).
(Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:23112293, PubMed:32686895, PubMed:31722778).
RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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