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Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427)


See all MAP2K3 antibodies
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Tested Data
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in ELISA
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in ELISA
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in WB
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in WB
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in WB
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in WB
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IF
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in FC
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in FC
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IHC
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IHC
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IHC
Mouse Anti-MAP2K3 Recombinant Antibody (CBT4027) (V2LY-0625-LY427) in IHC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4027
Antibody Isotype
IgG1
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human MAP2K3 (AA: 1-138) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
5606
UniProt ID
P46734
Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Biological Process
Cardiac muscle contractionIEA:Ensembl
Cellular response to lipopolysaccharideIEA:Ensembl
Cellular response to sorbitolIEA:Ensembl
Cellular response to vascular endothelial growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular senescenceTAS:Reactome
Heart developmentIEA:Ensembl
Inflammatory responseIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of hippo signalingManual Assertion Based On ExperimentIMP:FlyBase
p38MAPK cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of MAPK cascadeIEA:Ensembl
Positive regulation of nitric-oxide synthase biosynthetic processIEA:Ensembl
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokine productionIEA:Ensembl
Response to ischemiaIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytosol
Membrane
Nucleoplasm
Involvement in disease
Defects in MAP2K3 may be involved in colon cancer.
PTM
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669).
Phosphorylated by TAOK2 (PubMed:11279118).
(Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
More Infomation

Gu, K., Wang, T., Peng, L., & Zhao, Y. (2022). FIP-fve stimulates cell proliferation and enhances IL-2 release by activating MAP2K3/p38α (MAPK14) signaling pathway in Jurkat E6-1 cells. Frontiers in Nutrition, 9, 881924.

Bhargavi, M., Vhora, N., Lanka, G., Somadi, G., Kanth, S. S., Jain, A., & Potlapally, S. R. (2021). Homology modelling and virtual screening to explore potent inhibitors for MAP2K3 protein. Structural Chemistry, 32, 1039-1051.

Zhang, Y., Lu, W., Chen, Y., Lin, Y., Yang, X., Wang, H., & Liu, Z. (2021). The miR‐19b‐3p‐MAP2K3‐STAT3 feedback loop regulates cell proliferation and invasion in esophageal squamous cell carcinoma. Molecular Oncology, 15(5), 1566-1583.

Sawada, Y., Nakatsuji, T., Dokoshi, T., Kulkarni, N. N., Liggins, M. C., Sen, G., & Gallo, R. L. (2021). Cutaneous innate immune tolerance is mediated by epigenetic control of MAP2K3 by HDAC8/9. Science immunology, 6(59), eabe1935.

Qi, A., Wang, T., Li, W., Wang, Y., & Chai, Y. (2021). The effect of miR-21-5p on the MAP2K3 expressions and cellular apoptosis in the lung tissues of neonatal rats with hyperoxia-induced lung injuries. American Journal of Translational Research, 13(4), 2784.

Kim, Y. J., Jung, E., Shin, E., Hong, S. H., Jeong, H. S., Hur, G., ... & Kim, J. (2020). Genome-wide RNA interference screening reveals a COPI-MAP2K3 pathway required for YAP regulation. Proceedings of the National Academy of Sciences, 117(33), 19994-20003.

Gholipoorfeshkecheh, R., Agarwala, S., Krishnappa, S., Savitha, M. R., & Ramachandra, N. B. (2020). Whole-exome sequencing and homozygosity mapping identify variants in NCOR1 and MAP2K3 associated with non-syndromic congenital heart defects. Egyptian Journal of Medical Human Genetics, 21(1), 1-10.

Han, S., Wang, Y., Ma, J., Wang, Z., Wang, H. M. D., & Yuan, Q. (2020). Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop. Cell death & disease, 11(8), 713.

He, F., Xiao, Z., Yao, H., Li, S., Feng, M., Wang, W., ... & Wu, J. (2019). The protective role of microRNA-21 against coxsackievirus B3 infection through targeting the MAP2K3/P38 MAPK signaling pathway. Journal of translational medicine, 17(1), 1-11.

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For research use only. Not intended for any clinical use.

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