Mouse Anti-MAP3K12 Monoclonal Antibody (MM0514-9D8) (CBMAB-1023-YC)

Name: Mouse Anti-MAP3K12 Monoclonal Antibody (MM0514-9D8)
SKU: CBMAB-1023-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

MM0514-9D8

Application

FC, ICC, IF, IHC-P, WB

Immunogen

Recombinant Human DLK protein.

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 12

Introduction

MAP3K12 contains a leucine-zipper domain and is predominately expressed in neuronal cells, which is a member of the serine/threonine protein kinase family. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. MAP3K12 may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.

Entrez Gene ID

7786

UniProt ID

Q12852

Alternative Names

DLK; MUK; ZPK; ZPKP1; MEKK12

Function

Part of a non-canonical MAPK signaling pathway (PubMed:28111074).
Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074).
May be an activator of the JNK/SAPK pathway.

Biological Process

Histone phosphorylationISS:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
JNK cascadeBy SimilarityISS:UniProtKB
Negative regulation of motor neuron apoptotic processIEA:Ensembl
Peptidyl-serine phosphorylationISS:UniProtKB
Peptidyl-threonine phosphorylationISS:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of JUN kinase activityISS:ARUK-UCL
Positive regulation of protein kinase activityISS:ARUK-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Protein autophosphorylationBy SimilarityISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB

PTM

Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.

Tenenbaum, M., Plaisance, V., Boutry, R., Pawlowski, V., Jacovetti, C., Sanchez-Parra, C., ... & Abderrahmani, A. (2021). The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development. Cellular and Molecular Life Sciences, 78, 287-298.

Wan, W., Liu, G., Li, X., Liu, Y., Wang, Y., Pan, H., & Hu, J. (2021). MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease. Bioengineered, 12(2), 12678-12690.

Wang, C., Wan, H., Li, M., & Zhang, C. (2021). Celastrol attenuates ischemia/reperfusion-mediated memory dysfunction by downregulating AK005401/MAP3K12. Phytomedicine, 82, 153441.

Ochieng, J. K., Kundu, S. T., Bajaj, R., Leticia Rodriguez, B., Fradette, J. J., & Gibbons, D. L. (2020). MBIP (MAP3K12 binding inhibitory protein) drives NSCLC metastasis by JNK-dependent activation of MMPs. Oncogene, 39(43), 6719-6732.

Shi, Q., Yu, J., Li, P. A., Lu, X., & Wang, W. (2020). MicroRNA-337 participates in the progression of early-stage diabetic retinopathy by upregulating MAP3K12. Minerva Medica.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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