Human Recombinant Map3k12, Active protein, GST Tag (V2LY-0526-LY5430)

Name: Human Recombinant Map3k12, Active protein, GST Tag
SKU: V2LY-0526-LY5430
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant Map3k12, Active protein, GST Tag consist of Amino Acid: 1-520 and predicts a molecular mass of 94 kDa.

Molecule Mass

94 kDa

Sequence

Amino Acid: 1-520

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 12

Function

Part of a non-canonical MAPK signaling pathway (PubMed:28111074).
Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074).
May be an activator of the JNK/SAPK pathway.

Biological Process

Histone phosphorylationISS:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
JNK cascadeBy SimilarityISS:UniProtKB
Negative regulation of motor neuron apoptotic processIEA:Ensembl
Peptidyl-serine phosphorylationISS:UniProtKB
Peptidyl-threonine phosphorylationISS:UniProtKB
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of JUN kinase activityISS:ARUK-UCL
Positive regulation of protein kinase activityISS:ARUK-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Protein autophosphorylationBy SimilarityISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB

PTM

Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.

Tenenbaum, M., Plaisance, V., Boutry, R., Pawlowski, V., Jacovetti, C., Sanchez-Parra, C., ... & Abderrahmani, A. (2021). The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development. Cellular and Molecular Life Sciences, 78, 287-298.

Wan, W., Liu, G., Li, X., Liu, Y., Wang, Y., Pan, H., & Hu, J. (2021). MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease. Bioengineered, 12(2), 12678-12690.

Wang, C., Wan, H., Li, M., & Zhang, C. (2021). Celastrol attenuates ischemia/reperfusion-mediated memory dysfunction by downregulating AK005401/MAP3K12. Phytomedicine, 82, 153441.

Ochieng, J. K., Kundu, S. T., Bajaj, R., Leticia Rodriguez, B., Fradette, J. J., & Gibbons, D. L. (2020). MBIP (MAP3K12 binding inhibitory protein) drives NSCLC metastasis by JNK-dependent activation of MMPs. Oncogene, 39(43), 6719-6732.

Shi, Q., Yu, J., Li, P. A., Lu, X., & Wang, W. (2020). MicroRNA-337 participates in the progression of early-stage diabetic retinopathy by upregulating MAP3K12. Minerva Medica.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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