Mouse Anti-MAPK3 Recombinant Antibody (5E9) (CBMAB-AP2140LY)

Mitogen-Activated Protein Kinase 3

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1;

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIAC F4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Apoptotic processIEA:UniProtKB-KW
Bergmann glial cell differentiationIEA:Ensembl
BMP signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Cardiac neural crest cell development involved in heart developmentIEA:Ensembl
Cartilage developmentIEA:Ensembl
Caveolin-mediated endocytosisManual Assertion Based On ExperimentTAS:UniProtKB
Cell cycleIEA:UniProtKB-KW
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to amino acid starvationManual Assertion Based On ExperimentIDA:CAFA
Cellular response to cadmium ionManual Assertion Based On ExperimentIMP:CAFA
Cellular response to dopamineManual Assertion Based On ExperimentIMP:CAFA
Cellular response to mechanical stimulusManual Assertion Based On ExperimentIEP:UniProtKB
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:CAFA
Cellular response to tumor necrosis factorIEA:Ensembl
DNA damage induced protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
ERK1 and ERK2 cascadeIEA:Ensembl
Face developmentIEA:Ensembl
Interleukin-1-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Lipopolysaccharide-mediated signaling pathwayIEA:Ensembl
Lung morphogenesisIEA:Ensembl
MAPK cascade1 PublicationNAS:BHF-UCL
Negative regulation of apolipoprotein bindingIEA:Ensembl
Outer ear morphogenesisIEA:Ensembl
Peptidyl-tyrosine autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
PhosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cyclase activityManual Assertion Based On ExperimentIMP:CACAO
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:CAFA
Positive regulation of histone acetylationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of histone phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of macrophage chemotaxisManual Assertion Based On ExperimentIGI:ARUK-UCL
Positive regulation of macrophage proliferationManual Assertion Based On ExperimentIGI:ARUK-UCL
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomerase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere cappingManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere maintenance via telomeraseManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of xenophagyIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cellular pHIEA:Ensembl
Regulation of cytoskeleton organizationManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of DNA-binding transcription factor activityIEA:Ensembl
Regulation of early endosome to late endosome transportManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of Golgi inheritanceManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of ossificationIEA:Ensembl
Regulation of stress-activated MAPK cascadeManual Assertion Based On ExperimentTAS:UniProtKB
Response to epidermal growth factorManual Assertion Based On ExperimentIDA:UniProtKB
Response to exogenous dsRNAIEA:Ensembl
Sensory perception of painIEA:Ensembl
Stress-activated MAPK cascadeManual Assertion Based On ExperimentIDA:CAFA
Thymus developmentIEA:Ensembl
Thyroid gland developmentIEA:Ensembl
Trachea formationIEA:Ensembl
Transcription, DNA-templatedIEA:Ensembl

Cytoplasm
Nucleus
Membrane, caveola
Cell junction, focal adhesion
Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905).
PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity).

Phosphorylated upon KIT and FLT3 signaling (By similarity).
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.