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Rat Anti-MAPK4 (AA 1-126) Recombinant Antibody (CBFYM-1628) (CBMAB-M1795-FY)

This product is rat antibody that recognizes MAPK4. The antibody CBFYM-1628 can be used for immunoassay techniques such as: ELISA, WB.
See all MAPK4 antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse, Rat
Clone
CBFYM-1628
Antibody Isotype
IgG2a
Application
ELISA, WB

Basic Information

Immunogen
E. coli-derived recombinant human ERK4 (aa1-126)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, 5% trehalose
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-126

Target

Full Name
mitogen-activated protein kinase 4
Introduction
Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human5596
Mouse225724
Rat54268
UniProt ID
HumanP31152
MouseQ6P5G0
RatQ63454
Alternative Names
Mitogen-Activated Protein Kinase 4; Extracellular Signal-Regulated Kinase 4; MAP Kinase Isoform P63; EC 2.7.11.24; P63-MAPK; MAPK 4; PRKM4
Function
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity).
Biological Process
Cell cycleIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
Translocates to the cytoplasm following interaction with MAPKAPK5.
PTM
Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.

Ren, J., Zheng, S., Zhang, L., Liu, J., Cao, H., Wu, S., ... & Sun, J. (2023). MAPK4 predicts poor prognosis and facilitates the proliferation and migration of glioma through the AKT/mTOR pathway. Cancer Medicine.

Han, D., Wang, W., Jeon, J. H., Shen, T., Huang, X., Yi, P., ... & Yang, F. (2023). Cooperative activation of PDK1 and AKT by MAPK4 enhances cancer growth and resistance to therapy. PLoS biology, 21(8), e3002227.

Wang, W., Han, D., Cai, Q., Shen, T., Dong, B., Lewis, M. T., ... & Yang, F. (2022). MAPK4 promotes triple negative breast cancer growth and reduces tumor sensitivity to PI3K blockade. Nature communications, 13(1), 245.

Zeng, X., Jiang, S., Ruan, S., Guo, Z., Guo, J., Liu, M., ... & Dong, J. (2021). MAPK4 silencing together with a PARP1 inhibitor as a combination therapy in triple‑negative breast cancer cells. Molecular medicine reports, 24(2), 1-8.

Shen, T., Wang, W., Zhou, W., Coleman, I., Cai, Q., Dong, B., ... & Yang, F. (2021). MAPK4 promotes prostate cancer by concerted activation of androgen receptor and AKT. The Journal of clinical investigation, 131(4).

Tian, S., Lou, L., Tian, M., Lu, G., Tian, J., & Chen, X. (2020). MAPK4 deletion enhances radiation effects and triggers synergistic lethality with simultaneous PARP1 inhibition in cervical cancer. Journal of Experimental & Clinical Cancer Research, 39(1), 1-11.

He, J., Huang, Z., He, M., Liao, J., Zhang, Q., Wang, S., ... & Liu, A. (2020). Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas. Molecular cancer, 19(1), 1-17.

Feng, Y., Zhang, L., Wu, J., Khadka, B., Fang, Z., Gu, J., ... & Liu, J. (2019). CircRNA circ_0000190 inhibits the progression of multiple myeloma through modulating miR-767-5p/MAPK4 pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-17.

Wang, W., Shen, T., Dong, B., Creighton, C. J., Meng, Y., Zhou, W., ... & Yang, F. (2019). MAPK4 overexpression promotes tumor progression via noncanonical activation of AKT/mTOR signaling. The Journal of clinical investigation, 129(3), 1015-1029.

Raj, S., Sasidharan, S., Dubey, V. K., & Saudagar, P. (2019). Identification of lead molecules against potential drug target protein MAPK4 from L. donovani: An in-silico approach using docking, molecular dynamics and binding free energy calculation. PloS one, 14(8), e0221331.

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For research use only. Not intended for any clinical use.

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