Mouse Anti-MARCH1 (AA 1-99) Recombinant Antibody (CBFYM-1694) (CBMAB-M1861-FY)

Name: Mouse Anti-MARCH1 (AA 1-99) Recombinant Antibody (CBFYM-1694)
SKU: CBMAB-M1861-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1694

Application

S-ELISA, ELISA

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-99

More Infomation

Target

Full Name

membrane-associated ring finger (C3HC4) 1

Introduction

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases. MARCH proteins add ubiquitin to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex class II molecules and other glycoproteins by directing them to the late endosomal/lysosomal compartment.

Entrez Gene ID

55016

UniProt ID

Q8TCQ1

Alternative Names

Membrane Associated Ring-CH-Type Finger 1; Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase; Membrane-Associated RING Finger Protein 1; RING-Type E3 Ubiquitin Transferase MARCH1; Membrane-Associated RING-CH Protein I; RING Finger Protein 171; MARCH-I

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.

Biological Process

Antigen processing and presentation of peptide antigen via MHC class II Source: UniProtKB
Immune response Source: UniProtKB
Protein polyubiquitination Source: UniProtKB

Cellular Location

Plasma membrane
Cell membrane
Endosome
Late endosome membrane
Early endosome membrane
Lysosome
Lysosome membrane
Golgi apparatus
trans-Golgi network membrane
Other locations
Cytoplasmic vesicle membrane

PTM

Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins (By similarity).

Xu, Z., Liu, J., Liu, Z., & Zhang, H. (2022). MARCH1 as a novel immune-related prognostic biomarker that shapes an inflamed tumor microenvironment in lung adenocarcinoma. Frontiers in Oncology, 12, 1008753.

Villalón-Letelier, F., Farrukee, R., Londrigan, S. L., Brooks, A. G., & Reading, P. C. (2022). Isoforms of human MARCH1 differ in ability to restrict influenza A viruses due to differences in their N terminal cytoplasmic domain. Viruses, 14(11), 2549.

He, X., Liu, J., Xiao, X., Zhang, S., Wang, X., Liu, J., ... & Yang, P. (2022). Targeting psychological stress-steroid-MARCH1 signaling pathway promotes the efficacy of specific allergen immunotherapy. Theranostics, 12(18), 7717.

Castellanos, C. A., Ren, X., Gonzalez, S. L., Li, H. K., Schroeder, A. W., Liang, H. E., ... & Shin, J. S. (2021). Lymph node–resident dendritic cells drive TH2 cell development involving MARCH1. Science immunology, 6(64), eabh0707.

Yang, W., Su, J., Li, M., Li, T., Wang, X., Zhao, M., & Hu, X. (2021). Myricetin induces autophagy and cell cycle arrest of HCC by inhibiting MARCH1-regulated Stat3 and p38 MAPK signaling pathways. Frontiers in Pharmacology, 12, 709526.

Wu, J., Xia, L., Yao, X., Yu, X., Tumas, K. C., Sun, W., ... & Su, X. Z. (2020). The E3 ubiquitin ligase MARCH1 regulates antimalaria immunity through interferon signaling and T cell activation. Proceedings of the National Academy of Sciences, 117(28), 16567-16578.

Dai, H., Li, M., Yang, W., Sun, X., Wang, P., Wang, X., ... & Zhao, M. (2020). Resveratrol inhibits the malignant progression of hepatocellular carcinoma via MARCH1-induced regulation of PTEN/AKT signaling. Aging (Albany NY), 12(12), 11717.

Xie, L., Dai, H., Li, M., Yang, W., Yu, G., Wang, X., ... & Zhao, M. (2019). MARCH1 encourages tumour progression of hepatocellular carcinoma via regulation of PI3K‐AKT‐β‐catenin pathways. Journal of cellular and molecular medicine, 23(5), 3386-3401.

Xie, L., Li, M., Liu, D., Wang, X., Wang, P., Dai, H., ... & Zhao, M. (2019). Secalonic acid-F, a novel mycotoxin, represses the progression of hepatocellular carcinoma via MARCH1 regulation of the PI3K/AKT/β-catenin signaling pathway. Molecules, 24(3), 393.

Kang, Z., Jiang, E., Wang, K., Pan, C., Chen, H., Yan, H., ... & Lan, X. (2019). Goat membrane associated ring-CH-type finger 1 (MARCH1) mRNA expression and association with litter size. Theriogenology, 128, 8-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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