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  • Mouse Anti-MARCH5 Recombinant Antibody (CBFYM-0578)

Mouse Anti-MARCH5 Recombinant Antibody (CBFYM-0578) (CBMAB-M0707-FY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYM-0578
Application
ELISA
Specificity
Human
Antibody Isotype
IgG2a, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
membrane-associated ring finger (C3HC4) 5
Introduction
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 and DRP1 .
Entrez Gene ID
54708
UniProt ID
Q9NX47
Alternative Names
Membrane Associated Ring-CH-Type Finger 5; Mitochondrial Ubiquitin Ligase; Membrane-Associated Ring Finger (C3HC4) 5; Membrane-Associated RING Finger Protein 5; RING-Type E3 Ubiquitin Transferase MARCH5; Membrane-Associated RING-CH Protein V; Ring Finger Protein 153; MARCH-V
Function
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Biological Process
Negative regulation of cell aging Source: UniProtKB
Positive regulation of mitochondrial fission Source: UniProtKB
Protein autoubiquitination Source: UniProtKB
Protein localization to mitochondrion Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Regulation of mitochondrial fission Source: UniProtKB
Cellular Location
Endoplasmic reticulum
Endoplasmic reticulum membrane
Mitochondrion
Mitochondrion outer membrane
Note: Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).
PTM
Autoubiquitinated leading to degradation (short half-life).

Kitakata, H., Endo, J., Matsushima, H., Yamamoto, S., Ikura, H., Hirai, A., ... & Sano, M. (2021). MITOL/MARCH5 determines the susceptibility of cardiomyocytes to doxorubicin-induced ferroptosis by regulating GSH homeostasis. Journal of molecular and cellular cardiology, 161, 116-129.

Zheng, J., Chen, X., Liu, Q., Zhong, G., & Zhuang, M. (2021). Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy. Journal of Cell Biology, 221(1), e202103156.

Park, Y. J., Oanh, N. T. K., Heo, J., Kim, S. G., Lee, H. S., Lee, H., ... & Cho, H. (2020). Dual targeting of RIG-I and MAVS by MARCH5 mitochondria ubiquitin ligase in innate immunity. Cellular signalling, 67, 109520.

Shiiba, I., Takeda, K., Nagashima, S., & Yanagi, S. (2020). Overview of mitochondrial E3 ubiquitin ligase MITOL/MARCH5 from molecular mechanisms to diseases. International Journal of Molecular Sciences, 21(11), 3781.

Arai, S., Varkaris, A., Nouri, M., Chen, S., Xie, L., & Balk, S. P. (2020). MARCH5 mediates NOXA-dependent MCL1 degradation driven by kinase inhibitors and integrated stress response activation. Elife, 9, e54954.

Djajawi, T. M., Liu, L., Gong, J. N., Huang, A. S., Luo, M. J., Xu, Z., ... & van Delft, M. F. (2020). MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1: NOXA complex. Cell Death & Differentiation, 27(8), 2484-2499.

Li, Q., Yu, Z., Xiao, D., Wang, Y., Zhao, L., An, Y., & Gao, Y. (2020). Baicalein inhibits mitochondrial apoptosis induced by oxidative stress in cardiomyocytes by stabilizing MARCH5 expression. Journal of cellular and molecular medicine, 24(2), 2040-2051.

Haschka, M. D., Karbon, G., Soratroi, C., O’Neill, K. L., Luo, X., & Villunger, A. (2020). MARCH5-dependent degradation of MCL1/NOXA complexes defines susceptibility to antimitotic drug treatment. Cell Death & Differentiation, 27(8), 2297-2312.

Yoo, Y. S., Park, Y. J., Lee, H. S., Oanh, N. T. K., Cho, M. Y., Heo, J., ... & Cho, H. (2019). Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis. Cell death & disease, 10(12), 938.

Koyano, F., Yamano, K., Kosako, H., Kimura, Y., Kimura, M., Fujiki, Y., ... & Matsuda, N. (2019). Parkin‐mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO reports, 20(12), e47728.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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