Mouse Anti-MAT1A Recombinant Antibody (4D11) (CBMAB-A5315-LY)

Name: Mouse Anti-MAT1A Recombinant Antibody (4D11)
SKU: CBMAB-A5315-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4D11

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

MAT1A (AAH18359, 1 a.a. ~ 395 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

methionine adenosyltransferase I, alpha

Introduction

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq]

Entrez Gene ID

4143

UniProt ID

Q00266

Alternative Names

MAT; MATA1; SAMS; SAMS1

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Biological Process

Methionine catabolic process Source: UniProtKB
One-carbon metabolic process Source: UniProtKB-KW
Protein homotetramerization Source: UniProtKB
S-adenosylmethionine biosynthetic process Source: ComplexPortal

Cellular Location

Cytosol
Other locations
methionine adenosyltransferase complex

Involvement in disease

Methionine adenosyltransferase deficiency (MATD):
An inborn error of metabolism resulting in isolated hypermethioninemia. Most patients have no clinical abnormalities, although some neurologic symptoms may be present in rare cases with severe loss of methionine adenosyltransferase activity.

PTM

S-nitrosylation of Cys-120 inactivates the enzyme.
An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.

More Infomation

References

Li, Y., Hu, G., Huang, F., Chen, M., Chen, Y., Xu, Y., & Tong, G. (2023). MAT1A Suppression by the CTBP1/HDAC1/HDAC2 Transcriptional Complex Induces Immune Escape and Reduces Ferroptosis in Hepatocellular Carcinoma. Laboratory Investigation, 103(8), 100180.

Chen, P. M., Tsai, C. H., Huang, C. C., Hwang, H. H., Li, J. R., Liu, C. C., ... & Chiang, E. P. I. (2022). Downregulation of methionine cycle genes MAT1A and GNMT enriches protein-associated translation process and worsens hepatocellular carcinoma prognosis. International journal of molecular sciences, 23(1), 481.

Chen, X., Luo, J., Yang, L., Guo, Y., Fan, Y., Liu, J., ... & Xi, Q. (2022). miR-143-mediated responses to betaine supplement repress lipogenesis and hepatic gluconeogenesis by targeting MAT1a and MAPK11. Journal of Agricultural and Food Chemistry, 70(26), 7981-7992.

Panmanee, J., Antonyuk, S. V., & Hasnain, S. S. (2020). Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene. Acta Crystallographica Section D: Structural Biology, 76(6), 594-607.

Martin, K. A., Hum, N. R., Sebastian, A., He, W., Siddiqui, S., Ghosh, P. M., ... & Loots, G. G. (2019). Methionine adenosyltransferase 1a (MAT1A) enhances cell survival during chemotherapy treatment and is associated with drug resistance in bladder cancer PDX mice. International journal of molecular sciences, 20(20), 4983.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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