Human Recombinant MAT1A protein, GST-TEV Tag (V2LY-0526-LY5473)

Name: Human Recombinant MAT1A protein, GST-TEV Tag
SKU: V2LY-0526-LY5473
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST-TEV Tag

Protein Construction

This product is Human Recombinant MAT1A protein, GST-TEV Tag consist of Amino Acid: 1-395 and predicts a molecular mass of 65 kDa.

Molecule Mass

65 kDa

Sequence

Amino Acid: 1-395

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

methionine adenosyltransferase I, alpha

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Biological Process

Methionine catabolic process Source: UniProtKB
One-carbon metabolic process Source: UniProtKB-KW
Protein homotetramerization Source: UniProtKB
S-adenosylmethionine biosynthetic process Source: ComplexPortal

Cellular Location

Cytosol
Other locations
methionine adenosyltransferase complex

Involvement in disease

Methionine adenosyltransferase deficiency (MATD):
An inborn error of metabolism resulting in isolated hypermethioninemia. Most patients have no clinical abnormalities, although some neurologic symptoms may be present in rare cases with severe loss of methionine adenosyltransferase activity.

PTM

S-nitrosylation of Cys-120 inactivates the enzyme.
An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.

Li, Y., Hu, G., Huang, F., Chen, M., Chen, Y., Xu, Y., & Tong, G. (2023). MAT1A Suppression by the CTBP1/HDAC1/HDAC2 Transcriptional Complex Induces Immune Escape and Reduces Ferroptosis in Hepatocellular Carcinoma. Laboratory Investigation, 103(8), 100180.

Chen, P. M., Tsai, C. H., Huang, C. C., Hwang, H. H., Li, J. R., Liu, C. C., ... & Chiang, E. P. I. (2022). Downregulation of methionine cycle genes MAT1A and GNMT enriches protein-associated translation process and worsens hepatocellular carcinoma prognosis. International journal of molecular sciences, 23(1), 481.

Chen, X., Luo, J., Yang, L., Guo, Y., Fan, Y., Liu, J., ... & Xi, Q. (2022). miR-143-mediated responses to betaine supplement repress lipogenesis and hepatic gluconeogenesis by targeting MAT1a and MAPK11. Journal of Agricultural and Food Chemistry, 70(26), 7981-7992.

Panmanee, J., Antonyuk, S. V., & Hasnain, S. S. (2020). Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene. Acta Crystallographica Section D: Structural Biology, 76(6), 594-607.

Martin, K. A., Hum, N. R., Sebastian, A., He, W., Siddiqui, S., Ghosh, P. M., ... & Loots, G. G. (2019). Methionine adenosyltransferase 1a (MAT1A) enhances cell survival during chemotherapy treatment and is associated with drug resistance in bladder cancer PDX mice. International journal of molecular sciences, 20(20), 4983.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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