Rabbit Anti-MBD1 Recombinant Antibody (
10B10) (V2LY-0725-LY1370)

Name: Rabbit Anti-MBD1 Recombinant Antibody (10B10)
SKU: V2LY-0725-LY1370
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-MBD1 Recombinant Antibody (10B10) (V2LY-0725-LY1370) in IF

Immunofluorescence staining of MCF-7 cell with V2LY-0725-LY1370 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 498-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

10B10

Application

ELISA, IF

Immunogen

A synthesized peptide derived from human MBD1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MBD1

Entrez Gene ID

4152

UniProt ID

Q9UIS9

Function

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Biological Process

Negative regulation of transcription, DNA-templated Source: UniProtKB
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Nucleus
Nucleus matrix
Nucleus speckle
Other locations
Chromosome
Note: Nuclear, in a punctate pattern (PubMed:12711603). Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes (PubMed:10648624, PubMed:10454587).

PTM

Sumoylated, sumoylation may increase interaction with ATF7IP.

Mahana, Y., Ariyoshi, M., Nozawa, R. S., Shibata, S., Nagao, K., Obuse, C., & Shirakawa, M. (2023). Structural evidence for protein-protein interaction between the non-canonical methyl-CpG-binding domain of SETDB proteins and C11orf46. Structure.

Coelho, F. S., Sangi, S., Moraes, J. L., da Silva Santos, W., Gamosa, E. A., Fernandes, K. V. S., & Grativol, C. (2022). Methyl-CpG binding proteins (MBD) family evolution and conservation in plants. Gene, 824, 146404.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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