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Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47)


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Tested Data
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in WB
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in IHC
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in IHC
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in IHC
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in IHC
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in ELISA
Mouse Anti-MCAM Recombinant Antibody (CBT3996) (V2LY-0625-LY47) in ELISA

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3996
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
4162
UniProt ID
P43121
Function
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Biological Process
Anatomical structure morphogenesis Source: ProtInc
Angiogenesis Source: MGI
Cell adhesion Source: ProtInc
Glomerular filtration Source: UniProtKB
Positive regulation of cell migration Source: Ensembl
Vascular wound healing Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 24-559
Helical: 560-583
Cytoplasmic: 584-646
More Infomation

Pang, Y., Maxwell, E., Sindrewicz-Goral, P., Shapanis, A., Li, S., Morgan, M., & Yu, L. G. (2022). Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression. Biomolecules, 12(10), 1451.

Ikeguchi, R., Shimizu, Y., Kondo, A., Kanda, N., So, H., Kojima, H., & Kitagawa, K. (2021). Melanoma cell adhesion molecule expressing helper T cells in CNS inflammatory demyelinating diseases. Neurology-Neuroimmunology Neuroinflammation, 8(6).

Wang, Z., Xu, Q., Zhang, N., Du, X., Xu, G., & Yan, X. (2020). CD146, from a melanoma cell adhesion molecule to a signaling receptor. Signal transduction and targeted therapy, 5(1), 148.

Petersen, E. R., Ammitzbøll, C., Søndergaard, H. B., Oturai, A. B., Sørensen, P. S., Nilsson, A. C., ... & Sellebjerg, F. (2019). Expression of melanoma cell adhesion molecule-1 (MCAM-1) in natalizumab-treated multiple sclerosis. Journal of Neuroimmunology, 337, 577085.

Chen, Y., Sumardika, I. W., Tomonobu, N., Ruma, I. M. W., Kinoshita, R., Kondo, E., ... & Sakaguchi, M. (2019). Melanoma cell adhesion molecule is the driving force behind the dissemination of melanoma upon S100A8/A9 binding in the original skin lesion. Cancer Letters, 452, 178-190.

Čorić, M. (2019). Development and characterisation of monoclonal antibodies to tumour endothelial marker ‘melanoma cell adhesion molecule’(MCAM) and approaches to targeting MCAM on tumour vessels (Doctoral dissertation, University of Birmingham).

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For research use only. Not intended for any clinical use.

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