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Mouse Anti-MCL1 Recombinant Antibody (CBT871) (V2LY-0625-LY3213)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT871
Antibody Isotype
IgG
Application
WB, IHC-P, IF, ICC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human MCL-1 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
IF1:200-1:1,000
IHC1:200-1:1,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MCL1, BCL2 Family Apoptosis Regulator
Entrez Gene ID
4170
UniProt ID
Q07820
Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Biological Process
Cell fate determination Source: UniProtKB
Cellular homeostasis Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Negative regulation of anoikis Source: UniProtKB
Negative regulation of apoptotic process Source: BHF-UCL
Negative regulation of autophagy Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
Negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
Positive regulation of apoptotic process Source: ComplexPortal
Positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Regulation of apoptotic process Source: ComplexPortal
Regulation of response to DNA damage stimulus Source: MGI
Response to cytokine Source: MGI
Cellular Location
Mitochondrion
Cytoplasm
Nucleus
nucleoplasm
Other locations
Membrane
Note: Cytoplasmic, associated with mitochondria.
Topology
Helical: 328-348
PTM
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.
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For research use only. Not intended for any clinical use.

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