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Mouse Anti-MCM7 Recombinant Antibody (6C2) (CBMAB-A5352-LY)

The product is antibody recognizes MCM7. The antibody 6C2 immunoassay techniques such as: WB, ELISA.
See all MCM7 antibodies
Published Data
Fig.1 Immuno-co-expression images of Nek2 and MCM7/Ki-67 in NSCLC.
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Fig.2 Immunohistochemistry of serial sections of moderately differentiated lung adenocarcinoma showed the co-expressions of Nek2 and MCM7/Ki-67.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
6C2
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MCM7 (AAH09398, 1 a.a. ~ 389 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Minichromosome Maintenance Complex Component 7
Introduction
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
Entrez Gene ID
4176
UniProt ID
P33993
Alternative Names
CDABP0042; CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS-146
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Biological Process
Cell cycle Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to xenobiotic stimulus Source: Ensembl
DNA replication Source: ProtInc
DNA replication initiation Source: GO_Central
DNA strand elongation involved in DNA replication Source: GO_Central
DNA unwinding involved in DNA replication Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Regulation of DNA-dependent DNA replication initiation Source: ComplexPortal
Regulation of phosphorylation Source: UniProtKB
Cellular Location
Nucleus
Other locations
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
PTM
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Ubiquitinated by traip when forks converge following formation of DNA interstrand cross-links. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase.
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For research use only. Not intended for any clinical use.

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