Human MCM7 ELISA Kit (V2LY-0626-LY4502)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
81.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Minichromosome Maintenance Complex Component 7
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Biological Process
Cell cycle Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to xenobiotic stimulus Source: Ensembl
DNA replication Source: ProtInc
DNA replication initiation Source: GO_Central
DNA strand elongation involved in DNA replication Source: GO_Central
DNA unwinding involved in DNA replication Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Regulation of DNA-dependent DNA replication initiation Source: ComplexPortal
Regulation of phosphorylation Source: UniProtKB
Cellular Location
Nucleus
Other locations
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
PTM
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Ubiquitinated by traip when forks converge following formation of DNA interstrand cross-links. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase.

WANG, G., & YUN, H. (2023). Function of Minichromosome maintenance protein 7 (MCM7) in the Brain in a Rat Experimental Subarachnoid Hemorrhage Model.

Shamloo, N., Taghavi, N., Ahmadi, S., & Shalpoush, S. (2022). Immunohistochemical analysis of proliferating cell nuclear antigen and minichromosome maintenance complex component 7 in benign and malignant salivary gland tumors. Dental Research Journal, 19.

Schaefer, S., Doktor, T. K., Frederiksen, S. B., Chea, K., Hlavacova, M., Bruun, G. H., ... & Guerra, B. (2019). Down-regulation of CK2α correlates with decreased expression levels of DNA replication minichromosome maintenance protein complex (MCM) genes. Scientific Reports, 9(1), 14581.

Aporowicz, M., Czopnik, P., Kubicka, E., Piotrowska, A., Dziegiel, P., Bolanowski, M., & Domoslawski, P. (2019). Minichromosome maintenance proteins MCM-3, MCM-5, MCM-7, and Ki-67 as proliferative markers in adrenocortical tumors. Anticancer Research, 39(3), 1151-1159.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-MCM7 Recombinant Antibody (CAT#: CBMAB-AO734LY)

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Isotype control

For research use only. Not intended for any clinical use.

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