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Mouse Anti-MCM7 Recombinant Antibody (47DC141) (CBMAB-C6518-LY)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
47DC141
Application
IHC-P, IP, WB
Immunogen
BALB/C mice were injected with recombinant human CDC47 protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Minichromosome Maintenance Complex Component 7
Introduction
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Entrez Gene ID
4176
UniProt ID
P33993
Alternative Names
Minichromosome Maintenance Complex Component 7; Protein Phosphatase 1, Regulatory Subunit 104; CDC47 Homolog; P1.1-MCM3; CDC47; MCM2; MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 7; Mini
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Biological Process
Cell cycle Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to xenobiotic stimulus Source: Ensembl
DNA replication Source: ProtInc
DNA replication initiation Source: GO_Central
DNA strand elongation involved in DNA replication Source: GO_Central
DNA unwinding involved in DNA replication Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Regulation of DNA-dependent DNA replication initiation Source: ComplexPortal
Regulation of phosphorylation Source: UniProtKB
Cellular Location
Nucleus
Other locations
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
PTM
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Ubiquitinated by traip when forks converge following formation of DNA interstrand cross-links. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase.

WANG, G., & YUN, H. (2023). Function of Minichromosome maintenance protein 7 (MCM7) in the Brain in a Rat Experimental Subarachnoid Hemorrhage Model.

Shamloo, N., Taghavi, N., Ahmadi, S., & Shalpoush, S. (2022). Immunohistochemical analysis of proliferating cell nuclear antigen and minichromosome maintenance complex component 7 in benign and malignant salivary gland tumors. Dental Research Journal, 19.

Schaefer, S., Doktor, T. K., Frederiksen, S. B., Chea, K., Hlavacova, M., Bruun, G. H., ... & Guerra, B. (2019). Down-regulation of CK2α correlates with decreased expression levels of DNA replication minichromosome maintenance protein complex (MCM) genes. Scientific Reports, 9(1), 14581.

Aporowicz, M., Czopnik, P., Kubicka, E., Piotrowska, A., Dziegiel, P., Bolanowski, M., & Domoslawski, P. (2019). Minichromosome maintenance proteins MCM-3, MCM-5, MCM-7, and Ki-67 as proliferative markers in adrenocortical tumors. Anticancer Research, 39(3), 1151-1159.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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