Mouse Anti-MCPH1 Recombinant Antibody (5C9) (CBMAB-A5354-LY)

Name: Mouse Anti-MCPH1 Recombinant Antibody (5C9)
SKU: CBMAB-A5354-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5C9

Application

WB, ELISA

Immunogen

MCPH1 (NP_078872, 399 a.a. ~ 487 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MCPH1

Entrez Gene ID

79648

UniProt ID

Q8NEM0

Alternative Names

BRIT1; FLJ12847; MCT

Function

Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.

Biological Process

Bone development Source: Ensembl
Cerebral cortex development Source: Ensembl
Establishment of mitotic spindle orientation Source: Ensembl
Mitotic cell cycle Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Neuronal stem cell population maintenance Source: Ensembl
Protein localization to centrosome Source: Ensembl
Regulation of centrosome cycle Source: Ensembl
Regulation of chromosome condensation Source: Ensembl
Regulation of inflammatory response Source: Ensembl
Regulation of kinase activity Source: Ensembl

Cellular Location

Centrosome

Involvement in disease

Microcephaly 1, primary, autosomal recessive (MCPH1):
A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder. Some MCHP1 patients also present growth retardation, short stature, and misregulated chromosome condensation as indicated by a high number of prophase-like cells detected in routine cytogenetic preparations and poor-quality metaphase G-banding.

Alsolami, M., Aboalola, D., Malibari, D., Alghamdi, T., Alshekhi, W., Jad, H., ... & Alsiary, R. A. (2023). The emerging role of MCPH1/BRIT1 in carcinogenesis. Frontiers in Oncology, 13, 230.

Wang, Y., Zong, W., Sun, W., Chen, C., Wang, Z. Q., & Li, T. (2022). The central domain of MCPH1 controls development of the cerebral cortex and gonads in mice. Cells, 11(17), 2715.

Caraffi, S. G., Pollazzon, M., Farooq, M., Fatima, A., Larsen, L. A., Zuntini, R., ... & Garavelli, L. (2022). MCPH1: A Novel Case Report and a Review of the Literature. Genes, 13(4), 634.

Kristofova, M., Ori, A., & Wang, Z. Q. (2022). Multifaceted microcephaly-related gene MCPH1. Cells, 11(2), 275.

Khan, N. M., Masoud, M. S., Baig, S. M., Qasim, M., & Chang, J. (2022). Identification of Pathogenic Mutations in Primary Microcephaly-(MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in Consanguineous Pakistani Families. BioMed Research International, 2022.

Houlard, M., Cutts, E. E., Shamim, M. S., Godwin, J., Weisz, D., Aiden, A. P., ... & Nasmyth, K. (2021). MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface. Elife, 10, e73348.

Liu, X., Schneble-Löhnert, N., Kristofova, M., Qing, X., Labisch, J., Hofmann, S., ... & Wang, Z. Q. (2021). The N-terminal BRCT domain determines MCPH1 function in brain development and fertility. Cell Death & Disease, 12(2), 143.

Kristofova, M., & Wang, Z. Q. (2021). MCPH1, beyond its role deciding the brain size. Aging (Albany NY), 13(20), 23437.

Journiac, N., Gilabert-Juan, J., Cipriani, S., Benit, P., Liu, X., Jacquier, S., ... & Nardelli, J. (2020). Cell metabolic alterations due to Mcph1 mutation in microcephaly. Cell Reports, 31(2).

Shi, L., Luo, X., Jiang, J., Chen, Y., Liu, C., Hu, T., ... & Su, B. (2019). Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development. National Science Review, 6(3), 480-493.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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