Rabbit Anti-ME2 Recombinant Antibody (E1N3F) (CBMAB-CP1483-LY)

Name: Rabbit Anti-ME2 Recombinant Antibody (E1N3F)
SKU: CBMAB-CP1483-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

E1N3F

Application

WB, IF (ICC), FC

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu566 of human malic enzyme 2 protein.

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Malic Enzyme 2

Introduction

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Entrez Gene ID

Human	4200
Monkey	711129

UniProt ID

Human	P23368
Monkey	F6RIZ9

Alternative Names

Malic Enzyme 2; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2; NAD(+)-Dependent; Mitochondrial; NAD-Dependent Malic Enzyme; Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase; EC 1.1.1.38; EC 1.1.1; ODS1;

Function

NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.

Biological Process

Malate metabolic process Source: GO_Central
Pyruvate metabolic process Source: GO_Central
Regulation of NADP metabolic process Source: CACAO

Cellular Location

Mitochondrion matrix

Chen, K. C., Hsiao, I. H., Huang, Y. N., Chou, Y. T., Lin, Y. C., Hsieh, J. Y., ... & Hung, H. C. (2023). Targeting human mitochondrial NAD (P)+-dependent malic enzyme (ME2) impairs energy metabolism and redox state and exhibits antileukemic activity in acute myeloid leukemia. Cellular Oncology, 1-16.

Zhang, Z., & Du, W. (2022). Malic enzyme 2 as a therapeutic target for cancer: comments on ‘Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate’. Journal of Molecular Cell Biology, 14(4), mjac024.

Zhao, M., Yao, P., Mao, Y., Wu, J., Wang, W., Geng, C., ... & Jiang, P. (2022). Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate. Nature Metabolism, 4(2), 225-238.

Wang, Y. P., Sharda, A., Xu, S. N., Van Gastel, N., Man, C. H., Choi, U., ... & Scadden, D. T. (2021). Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. Cell Metabolism, 33(5), 1027-1041.

Zhang, S., Cheng, Z. M., Yu, J. L., Lu, K., Xu, S. J., Lu, Y., ... & Zhou, S. (2021). Malic enzyme 2 promotes the progression of hepatocellular carcinoma via increasing triglyceride production. Cancer Medicine, 10(19), 6795-6806.

You, D., Du, D., Zhao, X., Li, X., Ying, M., & Hu, X. (2021). Mitochondrial malic enzyme 2 promotes breast cancer metastasis via stabilizing HIF-1α under hypoxia. Chinese Journal of Cancer Research, 33(3), 308.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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