Rabbit Anti-ME3 Recombinant Antibody (CBFYM-1978) (CBMAB-M2153-FY)

This product is rabbit antibody that recognizes ME3. The antibody CBFYM-1978 can be used for immunoassay techniques such as: WB, IHC-P.
See all ME3 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Mouse, Rat, Human

Clone

CBFYM-1978

Antibody Isotype

IgG

Application

WB, IHC-P

Basic Information

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MALIC ENZYME 3

Introduction

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP-dependent isoform, a mitochondrial NADP-dependent isoform, and a mitochondrial NAD-dependent isoform. This gene encodes a mitochondrial NADP-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Entrez Gene ID

Human	10873
Mouse	109264
Rat	361602

UniProt ID

Human	Q16798
Mouse	Q8BMF3
Rat	F1M5N4

Alternative Names

Malic Enzyme 3; Malate Dehydrogenase (Oxaloacetate-Decarboxylating) (NADP(+)); Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial; EC 1.1.1.40; NADP-ME; Mitochondrial NADP(+)-Dependent Malic Enzyme 3

Function

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP+ as a cofactor (PubMed:7818469).

Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed:7818469).

Biological Process

Aerobic respiration Source: UniProtKB
Malate metabolic process Source: UniProtKB
Oxygen metabolic process Source: UniProtKB
Pyruvate metabolic process Source: UniProtKB

Cellular Location

Mitochondrion matrix

References

Rho, S. B., Byun, H. J., Kim, B. R., & Lee, C. H. (2023). Liver Kinase B1 Mediates Its Anti-Tumor Function by Binding to the N-Terminus of Malic Enzyme 3. Biomolecules & Therapeutics, 31(3), 330.

Grell, T. A., Mason, M., Thompson, A. A., Gomez-Tamayo, J. C., Riley, D., Wagner, M. V., ... & Yu, X. (2022). Structures of human Malic Enzyme 3. bioRxiv, 2022-08.

Grell, T. A., Mason, M., Thompson, A. A., Gómez-Tamayo, J. C., Riley, D., Wagner, M. V., ... & Yu, X. (2022). Integrative structural and functional analysis of human malic enzyme 3: A potential therapeutic target for pancreatic cancer. Heliyon, 8(12), e12392.

Sheth, G., Shah, S. R., Sengupta, P., Jarag, T., Chimanwala, S., Sairam, K. V., ... & Chitturi, T. R. (2022). In the Quest for Potent and Selective Malic Enzyme 3 Inhibitors for the Treatment of Pancreatic Ductal Adenocarcinoma. ACS Medicinal Chemistry Letters, 14(1), 41-50.

You, D., Du, D., Li, X., & Hu, X. (2021). Expression of Malic Enzyme 3 in Breast Cancer and Precancerous Lesions: a Promising Novel Biomarker for Carcinogenesis and Prognosis.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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