Mouse Anti-MEAK7 Recombinant Antibody (12B1) (CBMAB-K2210-LY)

This product is antibody recognizes TLDC1. The antibody 12B1 immunoassay techniques such as: WB, IHC, IF.
See all MEAK7 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

12B1

Antibody Isotype

IgG1

Application

WB, IHC, IF

Basic Information

Immunogen

Full length human recombinant protein of human KIAA1609 (NP_065998) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.98 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

MTOR Associated Protein, Eak-7 Homolog

Introduction

TLDC1 (TBC/LysM-Associated Domain Containing 1) is a Protein Coding gene. An important paralog of this gene is OXR1.

Entrez Gene ID

57707

UniProt ID

Q6P9B6

Alternative Names

TBC/LysM-Associated Domain Containing 1; KIAA1609; TBC/LysM-Associated Domain-Containing Protein 1; TLD Domain Containing 1; TLD Domain-Containing Protein 1;

Function

Activates an alternative mTOR signaling through RPS6KB2 activation and EIF4EBP1 repression to regulate cell proliferation and migration (PubMed:29750193).

Recruits MTOR at the lysosome, essential for MTOR signaling at the lysosome (PubMed:29750193).

Biological Process

Negative regulation of oxidative stress-induced neuron death Source: Ensembl
Positive regulation of protein localization to lysosome Source: UniProtKB
Regulation of cell migration Source: UniProtKB
Regulation of cell population proliferation Source: UniProtKB
Response to amino acid Source: UniProtKB
Response to insulin Source: UniProtKB
Response to nutrient levels Source: UniProtKB
TOR signaling Source: UniProtKB

Cellular Location

Lysosome
Cytoplasm
Other locations
Membrane

References

Wang, L., Wu, D., Robinson, C. V., & Fu, T. M. (2022). Identification of mEAK-7 as a human V-ATPase regulator via cryo-EM data mining. Proceedings of the National Academy of Sciences, 119(35), e2203742119.

Wang, R., Qin, Y., Xie, X. S., & Li, X. (2022). Molecular basis of mEAK7-mediated human V-ATPase regulation. Nature communications, 13(1), 3272.

Tan, Y. Z., Abbas, Y. M., Wu, J. Z., Wu, D., Keon, K. A., Hesketh, G. G., ... & Rubinstein, J. L. (2022). CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7. Life Science Alliance, 5(11).

Tan, Y. Z., Abbas, Y. M., Wu, J. Z., Wu, D., Hesketh, G. G., Bueler, S. A., ... & Rubinstein, J. L. (2021). Structure of mammalian V-ATPase with the TLDc domain protein mEAK7 bound. BioRxiv.

Mendonça, D. B., Nguyen, J. T., Haidar, F., Fox, A. L., Ray, C., Amatullah, H., ... & Krebsbach, P. H. (2020). MicroRNA-1911-3p targets mEAK-7 to suppress mTOR signaling in human lung cancer cells. Heliyon, 6(12).

Nguyen, J. T., Haidar, F. S., Fox, A. L., Ray, C., Mendonça, D. B., Kim, J. K., & Krebsbach, P. H. (2020). Abstract B05: mEAK-7 supports self-renewal and radioresistance in metastatic cancer. Molecular Cancer Research, 18(10_Supplement), B05-B05.

Nguyen, J. T., Haidar, F. S., Fox, A. L., Ray, C., Mendonça, D. B., Kim, J. K., & Krebsbach, P. H. (2019). mEAK-7 forms an alternative mTOR complex with DNA-PKcs in human cancer. Iscience, 17, 190-207.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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