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Rabbit Anti-MECOM Recombinant Antibody (C50E12) (CBMAB-CP0654-LY)

The product is antibody recognizes Evi-1. The antibody C50E12 immunoassay techniques such as: WB,IP,IF (ICC),FC.
See all MECOM antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
C50E12
Antibody Isotype
IgG
Application
WB, IP, IF (ICC), FC

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu56 of human Evi-1.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MDS1 and EVI1 complex locus
Entrez Gene ID
2122
UniProt ID
Q03112
Function
Isoform 1:
Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.

Isoform 7:
Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell differentiation Source: UniProtKB-KW
Hematopoietic stem cell proliferation Source: UniProtKB
Heterochromatin organization Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Negative regulation of programmed cell death Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Nucleus speckle
Cytoplasm
Involvement in disease
A chromosomal aberration involving EVI1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with RUNX1/AML1.
Radioulnar synostosis with amegakaryocytic thrombocytopenia 2 (RUSAT2):
An autosomal dominant disease characterized by proximal fusion of the radius and ulna resulting in extremely limited pronation and supination of the forearm, and congenital thrombocytopenia that progresses to pancytopenia.
A chromosomal aberration involving MDS1 is found in a form of acute myeloid leukemia (AML). Translocation t(3;21) with AML1.
PTM
Phosphorylated.
May be acetylated by CREBBP and KAT2B.

Voit, R. A., & Sankaran, V. G. (2023). MECOM Deficiency: from Bone Marrow Failure to Impaired B-Cell Development. Journal of Clinical Immunology, 43(6), 1052-1066.

Tang, Z., Wang, W., Yang, S., El Achi, H., Fang, H., Nahmod, K. A., ... & Tang, G. (2023). 3q26. 2/MECOM Rearrangements by Pericentric Inv (3): Diagnostic Challenges and Clinicopathologic Features. Cancers, 15(2), 458.

Chen, Y., Ying, Y., Wang, M., Ma, C., Jia, M., Shi, L., ... & Shu, X. S. (2023). A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer. Cell Death & Disease, 14(1), 8.

McCracken, I. R., Dobie, R., Bennett, M., Passi, R., Beqqali, A., Henderson, N. C., ... & Baker, A. H. (2022). Mapping the developing human cardiac endothelium at single-cell resolution identifies MECOM as a regulator of arteriovenous gene expression. Cardiovascular research, 118(14), 2960-2972.

Huber, S., Haferlach, T., Meggendorfer, M., Hutter, S., Hoermann, G., Baer, C., ... & Haferlach, C. (2022). SF3B1 mutations in AML are strongly associated with MECOM rearrangements and may be indicative of an MDS pre-phase. Leukemia, 36(12), 2927-2930.

Niihori, T., Tanoshima, R., Sasahara, Y., Sato, A., Irie, M., Saito-Nanjo, Y., ... & Aoki, Y. (2022). Phenotypic heterogeneity in individuals with MECOM variants in 2 families. Blood Advances, 6(18), 5257-5261.

Bleu, M., Mermet-Meillon, F., Apfel, V., Barys, L., Holzer, L., Bachmann Salvy, M., ... & Galli, G. G. (2021). PAX8 and MECOM are interaction partners driving ovarian cancer. Nature communications, 12(1), 2442.

Backx, E., Wauters, E., Baldan, J., Van Bulck, M., Michiels, E., Heremans, Y., ... & Rooman, I. (2021). MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions. Cell Death & Differentiation, 28(9), 2601-2615.

Ottema, S., Mulet-Lazaro, R., Beverloo, H. B., Erpelinck, C., van Herk, S., van der Helm, R., ... & Delwel, R. (2020). Atypical 3q26/MECOM rearrangements genocopy inv (3)/t (3; 3) in acute myeloid leukemia. Blood, The Journal of the American Society of Hematology, 136(2), 224-234.

Liu, K., & Tirado, C. A. (2019). MECOM: A Very Interesting Gene Involved also in Lymphoid Malignancies. Journal of the Association of Genetic Technologists, 45(3).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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