Rabbit Anti-MECOM Recombinant Antibody (S.446.4) (CBMAB-E2196-FY)

Name: Rabbit Anti-MECOM Recombinant Antibody (S.446.4)
SKU: CBMAB-E2196-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

S.446.4

Application

FC, ICC, IF, IP, WB

Immunogen

Synthetic peptide corresponding to residues surrounding Glu56 of human Evi-1

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10mM Sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MDS1 and EVI1 complex locus

Introduction

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.

Entrez Gene ID

2122

UniProt ID

Q03112

Alternative Names

MDS1 And EVI1 Complex Locus; Ecotropic Virus Integration Site 1 Protein Homolog; Myelodysplasia Syndrome-Associated Protein 1; PR Domain 3; EVI1; MDS1; MDS1 And EVI1 Complex Locus Protein EVI1; MDS1 And EVI1 Complex Locus Protein MDS1; Ecotropic Viral Integration Site 1; Myelodysplasia Syndrome 1 Protein; Myelodysplasia Syndrome 1

Function

Isoform 1:
Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.

Isoform 7:
Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell differentiation Source: UniProtKB-KW
Hematopoietic stem cell proliferation Source: UniProtKB
Heterochromatin organization Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Negative regulation of programmed cell death Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus
Nucleus speckle
Cytoplasm

Involvement in disease

A chromosomal aberration involving EVI1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with RUNX1/AML1.
Radioulnar synostosis with amegakaryocytic thrombocytopenia 2 (RUSAT2):
An autosomal dominant disease characterized by proximal fusion of the radius and ulna resulting in extremely limited pronation and supination of the forearm, and congenital thrombocytopenia that progresses to pancytopenia.
A chromosomal aberration involving MDS1 is found in a form of acute myeloid leukemia (AML). Translocation t(3;21) with AML1.

PTM

Phosphorylated.
May be acetylated by CREBBP and KAT2B.

Voit, R. A., & Sankaran, V. G. (2023). MECOM Deficiency: from Bone Marrow Failure to Impaired B-Cell Development. Journal of Clinical Immunology, 43(6), 1052-1066.

Tang, Z., Wang, W., Yang, S., El Achi, H., Fang, H., Nahmod, K. A., ... & Tang, G. (2023). 3q26. 2/MECOM Rearrangements by Pericentric Inv (3): Diagnostic Challenges and Clinicopathologic Features. Cancers, 15(2), 458.

Chen, Y., Ying, Y., Wang, M., Ma, C., Jia, M., Shi, L., ... & Shu, X. S. (2023). A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer. Cell Death & Disease, 14(1), 8.

McCracken, I. R., Dobie, R., Bennett, M., Passi, R., Beqqali, A., Henderson, N. C., ... & Baker, A. H. (2022). Mapping the developing human cardiac endothelium at single-cell resolution identifies MECOM as a regulator of arteriovenous gene expression. Cardiovascular research, 118(14), 2960-2972.

Huber, S., Haferlach, T., Meggendorfer, M., Hutter, S., Hoermann, G., Baer, C., ... & Haferlach, C. (2022). SF3B1 mutations in AML are strongly associated with MECOM rearrangements and may be indicative of an MDS pre-phase. Leukemia, 36(12), 2927-2930.

Niihori, T., Tanoshima, R., Sasahara, Y., Sato, A., Irie, M., Saito-Nanjo, Y., ... & Aoki, Y. (2022). Phenotypic heterogeneity in individuals with MECOM variants in 2 families. Blood Advances, 6(18), 5257-5261.

Bleu, M., Mermet-Meillon, F., Apfel, V., Barys, L., Holzer, L., Bachmann Salvy, M., ... & Galli, G. G. (2021). PAX8 and MECOM are interaction partners driving ovarian cancer. Nature communications, 12(1), 2442.

Backx, E., Wauters, E., Baldan, J., Van Bulck, M., Michiels, E., Heremans, Y., ... & Rooman, I. (2021). MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions. Cell Death & Differentiation, 28(9), 2601-2615.

Ottema, S., Mulet-Lazaro, R., Beverloo, H. B., Erpelinck, C., van Herk, S., van der Helm, R., ... & Delwel, R. (2020). Atypical 3q26/MECOM rearrangements genocopy inv (3)/t (3; 3) in acute myeloid leukemia. Blood, The Journal of the American Society of Hematology, 136(2), 224-234.

Liu, K., & Tirado, C. A. (2019). MECOM: A Very Interesting Gene Involved also in Lymphoid Malignancies. Journal of the Association of Genetic Technologists, 45(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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