Rabbit Anti-MED12 Recombinant Antibody (CBR084G) (CBMAB-BT061LY)

Name: Rabbit Anti-MED12 Recombinant Antibody (CBR084G)
SKU: CBMAB-BT061LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBR084G

Application

WB, IP, IHC, ICC

Immunogen

aa 2127-2177 (C-term)

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

mediator complex subunit 12

Introduction

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]

Entrez Gene ID

Human	9968
Mouse	59024

UniProt ID

Human	Q93074
Mouse	A2AGH6

Alternative Names

Mediator Complex Subunit 12; Thyroid Hormone Receptor-Associated Protein Complex 230 KDa Component; Trinucleotide Repeat-Containing Gene 11 Protein; Activator-Recruited Cofactor 240 KDa Component; OPA-Containing Protein; CAG Repeat Protein 45; TRAP230; TNRC11; ARC240; CAGH45; HOPA; Mediator Of RNA Polymerase II Transcription, Subunit 12 Homolog (S. Cerevisiae); Trinucleotide Repeat Containing 11 (THR-Associated Protein, 230 KDa Subunit); Trinucleotide Repeat Containing 11 (THR-Associated Protein, 230kDa Subunit);

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

Biological Process

Axis elongation involved in somitogenesis Source: Ensembl
Canonical Wnt signaling pathway Source: Ensembl
Embryonic brain development Source: Ensembl
Embryonic neurocranium morphogenesis Source: Ensembl
Endoderm development Source: Ensembl
Heart development Source: Ensembl
Neural tube closure Source: Ensembl
Oligodendrocyte development Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: MGI
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Post-anal tail morphogenesis Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Schwann cell development Source: Ensembl
Spinal cord development Source: Ensembl
Stem cell population maintenance Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: Ensembl

Cellular Location

Nucleus

Involvement in disease

Opitz-Kaveggia syndrome (OKS):
X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Intellectual developmental disorder, X-linked, syndromic, Lujan-Fryns type (MRXSLF):
A disorder characterized by tall stature with asthenic habitus, macrocephaly, a tall narrow face, maxillary hypoplasia, a high narrow palate with dental crowding, a small or receding chin, long hands with hyperextensible digits, hypernasal speech, hypotonia, mild-to-moderate mental retardation, behavioral aberrations and dysgenesis of the corpus callosum.
Ohdo syndrome, X-linked (OHDOX):
A syndrome characterized by mental retardation, feeding problems, and distinctive facial appearance with coarse facial features, severe blepharophimosis, ptosis, a bulbous nose, micrognathia and a small mouth. Dental hypoplasia and deafness can be considered as common manifestations of the syndrome. Male patients show cryptorchidism and scrotal hypoplasia.

Amendola, I. L. S., Spann, M., Segars, J., & Singh, B. (2023). The Mediator Complex Subunit 12 (MED-12) Gene and Uterine Fibroids: a Systematic Review. Reproductive Sciences, 1-18.

Gonzalez, C. G., Akula, S., & Burleson, M. (2022). The role of mediator subunit 12 in tumorigenesis and cancer therapeutics. Oncology letters, 23(3), 1-8.

Zhang, S., Liu, F., Halfmann, P., Behrens, R. T., Liu, P., Mcilwain, S. J., ... & Xu, W. (2022). Mediator complex subunit 12 is a gatekeeper of SARS-CoV-2 infection in breast cancer cells. Genes & Diseases, 9(1), 5.

Corachán, A., Trejo, M. G., Carbajo-García, M. C., Monleón, J., Escrig, J., Faus, A., ... & Ferrero, H. (2021). Vitamin D as an effective treatment in human uterine leiomyomas independent of mediator complex subunit 12 mutation. Fertility and Sterility, 115(2), 512-521.

Zhang, S., O’Regan, R., & Xu, W. (2020). The emerging role of mediator complex subunit 12 in tumorigenesis and response to chemotherapeutics. Cancer, 126(5), 939-948.

Poot, M. (2019). Mutations in mediator complex genes CDK8, MED12, MED13, and MEDL13 mediate overlapping developmental syndromes. Molecular Syndromology, 10(5), 239-242.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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