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Mouse Anti-MED12 Recombinant Antibody (CBYJT-1461) (CBMAB-T0492-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to MED12 (Mediator Complex Subunit 12). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all MED12 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1461
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
mediator complex subunit 12
Introduction
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is necessary for activating CDK8 kinase. Defects in MED12 cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.
Entrez Gene ID
9968
UniProt ID
Q93074
Alternative Names
Mediator Complex Subunit 12; Thyroid Hormone Receptor-Associated Protein Complex 230 KDa Component; Trinucleotide Repeat-Containing Gene 11 Protein; Activator-Recruited Cofactor 240 KDa Component; OPA-Containing Protein; CAG Repeat Protein 45; TRAP230; TNRC11; ARC240; CAGH45; HOPA; Mediator Of RNA Polymerase II Transcription, Subunit 12 Homolog (S. Cerevisiae); Trinucleotide Repeat Containing 11 (THR-Associated Protein, 230 KDa Subunit); Trinucleotide Repeat Containing 11 (THR-Associated Protein, 230kDa Subunit)
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Biological Process
Axis elongation involved in somitogenesis Source: Ensembl
Canonical Wnt signaling pathway Source: Ensembl
Embryonic brain development Source: Ensembl
Embryonic neurocranium morphogenesis Source: Ensembl
Endoderm development Source: Ensembl
Heart development Source: Ensembl
Neural tube closure Source: Ensembl
Oligodendrocyte development Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: MGI
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Post-anal tail morphogenesis Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Schwann cell development Source: Ensembl
Spinal cord development Source: Ensembl
Stem cell population maintenance Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Opitz-Kaveggia syndrome (OKS):
X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Intellectual developmental disorder, X-linked, syndromic, Lujan-Fryns type (MRXSLF):
A disorder characterized by tall stature with asthenic habitus, macrocephaly, a tall narrow face, maxillary hypoplasia, a high narrow palate with dental crowding, a small or receding chin, long hands with hyperextensible digits, hypernasal speech, hypotonia, mild-to-moderate mental retardation, behavioral aberrations and dysgenesis of the corpus callosum.
Ohdo syndrome, X-linked (OHDOX):
A syndrome characterized by mental retardation, feeding problems, and distinctive facial appearance with coarse facial features, severe blepharophimosis, ptosis, a bulbous nose, micrognathia and a small mouth. Dental hypoplasia and deafness can be considered as common manifestations of the syndrome. Male patients show cryptorchidism and scrotal hypoplasia.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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