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Mouse Anti-MED15 Recombinant Antibody (6F4) (CBMAB-BR349LY)

The product is antibody recognizes MED15. The antibody 6F4 immunoassay techniques such as: FC, IF, ICC, WB.
See all MED15 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6F4
Antibody Isotype
IgG1
Application
FC: 1-3 μg/1x10 cells, IF: 1:1000 dilution, ICC: 1:200 dilution, WB: 0.1-0.5 μg/ml

Basic Information

Immunogen
E. coli-derived human MED15 recombinant protein (Position: M1-A285).
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Preservative
0.05 mg sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mediator complex subunit 15
Introduction
The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Entrez Gene ID
51586
UniProt ID
Q96RN5
Alternative Names
Mediator of RNA polymerase II transcription subunit 15; Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine; Q-rich-associated protein; PC2 glutamine; Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein; MED15; ARC105; CTG7A; PCQAP; TIG1; TNRC7
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
Biological Process
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Stem cell population maintenance Source: Ensembl
Cellular Location
Nucleus
Cytoplasm
PTM
Ubiquitinated by TRIM11, leading to proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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