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Mouse Anti-MED16 Recombinant Antibody (CBYJT-1463) (CBMAB-T0494-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to MED16 (Mediator Complex Subunit 16). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, WB.
See all MED16 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1463
Antibody Isotype
IgG2a, κ
Application
ELISA, ICC, IF, WB

Basic Information

Immunogen
MED16 (AAH17282, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-110

Target

Full Name
Mediator Complex Subunit 16
Introduction
MED16 (Mediator Complex Subunit 16) is a Protein Coding gene. Among its related pathways are PEDF Induced Signaling and Thyroid hormone signaling pathway. Gene Ontology (GO) annotations related to MED16 include receptor activity and transcription cofactor activity.
Entrez Gene ID
10025
UniProt ID
Q9Y2X0
Alternative Names
Mediator Complex Subunit 16; Thyroid Hormone Receptor-Associated Protein Complex 95 KDa Component; Vitamin D3 Receptor-Interacting Protein Complex 92 KDa Component; Thyroid Hormone Receptor-Associated Protein 5; TRAP95; THRAP5
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Biological Process
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Transcription by RNA polymerase II Source: ProtInc
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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