Mouse Anti-MED17 Recombinant Antibody (CBFYM-1999) (CBMAB-M2177-FY)

Name: Mouse Anti-MED17 Recombinant Antibody (CBFYM-1999)
SKU: CBMAB-M2177-FY
Rating: 5 (1 reviews)

See all MED17 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1999

Application

Immunogen

Recombinant human Cofactor Required for Sp1 Transcriptional Activation Subunit 6, 77kD

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mediator Complex Subunit 17

Introduction

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Entrez Gene ID

9440

UniProt ID

Q9NVC6

Alternative Names

Mediator Complex Subunit 17; Cofactor Required For Sp1 Transcriptional Activation, Subunit 6, 77kDa; Thyroid Hormone Receptor-Associated Protein Complex 80 KDa Component; Vitamin D3 Receptor-Interacting Protein Complex 80 KDa Component; Activator-Recruited Cofactor 77 KDa Component; Transcriptional Coactivator CRSP77; CRSP Complex Subunit 6; DRIP80; TRAP80

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Biological Process

Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: MGI
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: MGI
RNA polymerase II preinitiation complex assembly Source: ComplexPortal
Transcription initiation from RNA polymerase II promoter Source: ProtInc

Cellular Location

Nucleus

Involvement in disease

Microcephaly, postnatal progressive, with seizures and brain atrophy (MCPHSBA):
A disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth.

Schiano, C., Luongo, L., Maione, S., & Napoli, C. (2023). Mediator complex in neurological disease. Life Sciences, 121986.

Agrawal, R., Singh, A., Giri, J., Magyar, Z., & Thakur, J. K. (2023). MEDIATOR SUBUNIT17 is required for transcriptional optimization of root system architecture in Arabidopsis. Plant Physiology, 192(2), 1548-1568.

Giustozzi, M., Freytes, S. N., Jaskolowski, A., Lichy, M., Mateos, J., Falcone Ferreyra, M. L., ... & Casati, P. (2022). Arabidopsis mediator subunit 17 connects transcription with DNA repair after UV‐B exposure. The Plant Journal, 110(4), 1047-1067.

Tourigny, J. P., Schumacher, K., Saleh, M. M., Devys, D., & Zentner, G. E. (2021). Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae. Genetics, 217(3), iyaa042.

Sierecki, E. (2020, March). The Mediator complex and the role of protein-protein interactions in the gene regulation machinery. In Seminars in Cell & Developmental Biology (Vol. 99, pp. 20-30). Academic Press.

Verger, A., Monté, D., & Villeret, V. (2019). Twenty years of Mediator complex structural studies. Biochemical Society Transactions, 47(1), 399-410.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MED17 Recombinant Antibody (CBFYM-1999)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-MED17 Recombinant Antibody (4D4) (CAT#: CBMAB-C6788-LY)

Mouse Anti-MED17 Monoclonal Antibody (2215C3a) (CAT#: CBMAB-1656-YC)

Mouse Anti-MED17 Recombinant Antibody (CBFYM-2000) (CAT#: CBMAB-M2178-FY)

Mouse Anti-MED17 (AA 6-77) Recombinant Antibody (CBFYC-2944) (CAT#: CBMAB-C3020-FY)

Mouse Anti-MED17 Recombinant Antibody (4D4) (CAT#: CBMAB-A1866-LY)

Mouse Anti-MED17 (AA 551-652) Recombinant Antibody (CBFYM-0490) (CAT#: CBMAB-M0618-FY)

Mouse Anti-MED17 Recombinant Antibody (CBYY-C2878) (CAT#: CBMAB-C4321-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry