Mouse Anti-MEPE (AA 18-525) Recombinant Antibody (CBFYM-2077) (CBMAB-M2258-FY)

This product is mouse antibody that recognizes MEPE. The antibody CBFYM-2077 can be used for immunoassay techniques such as: WB.
See all MEPE antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2077

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MEPE/OF45, Pro18-Asp525

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 18-525

Target

Full Name

MATRIX EXTRACELLULAR PHOSPHOGLYCOPROTEIN

Introduction

This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

56955

UniProt ID

Q9NQ76

Alternative Names

Matrix Extracellular Phosphoglycoprotein; Matrix, Extracellular Phosphoglycoprotein With ASARM Motif (Bone); Osteoblast/Osteocyte Factor 45; OF45

Function

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption (PubMed:14962809, PubMed:19005008).

Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed:18162525, PubMed:19998030, PubMed:22766095).

Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity).

Promotes dental pulp stem cell proliferation and differentiation (PubMed:22341070).

Biological Process

Biomineral tissue development Source: GO_Central
Skeletal system development Source: ProtInc

Cellular Location

Extracellular matrix

PTM

Phosphorylated on serine residues in the ASARM motif (in vitro) by FAM20C; the phosphorylation is important for the inhibition of bone mineralization (PubMed:19998030, PubMed:22582013).
Cleaved by CTSB/cathepsin B; the cleavage is blocked by metalloprotease PHEX.

References

Donmez, B. O., Karagur, E. R., Donmez, A. C., Choi, J., & Akkus, O. (2022). Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. Molecular Medicine Reports, 26(6), 1-11.

BEREKET, A. (2020). Fibroblast Growth Factor-23 and Matrix Extracellular Phosphoglycoprotein Levels in Healthy Children and, Pregnant and Puerperal Women.

Ozsen, A., Furman, A., Guran, T., Bereket, A., & Turan, S. (2020). Fibroblast Growth Factor-23 and Matrix Extracellular Phosphoglycoprotein Levels in Healthy Children and, Pregnant and Puerperal Women. Hormone Research in Paediatrics, 92(5), 302-310.

Christensen, B., Schytte, G. N., Scavenius, C., Enghild, J. J., McKee, M. D., & Sørensen, E. S. (2020). FAM20C‐Mediated Phosphorylation of MEPE and Its Acidic Serine‐and Aspartate‐Rich Motif. JBMR plus, 4(8), e10378.

Minamizaki, T., Sakurai, K., Hayashi, I., Toshishige, M., Yoshioka, H., Kozai, K., & Yoshiko, Y. (2020). Active sites of human MEPE-ASARM regulating bone matrix mineralization. Molecular and Cellular Endocrinology, 517, 110931.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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