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Mouse Anti-MESP1 Recombinant Antibody (4B4) (CBMAB-A5435-LY)

The product is antibody recognizes MESP1. The antibody 4B4 immunoassay techniques such as: WB, ELISA.
See all MESP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4B4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MESP1 (NP_061140.1, 1 a.a. ~ 63 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Mesoderm Posterior BHLH Transcription Factor 1
Entrez Gene ID
55897
UniProt ID
Q9BRJ9
Alternative Names
MGC10676; bHLHc5
Function
Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways (By similarity).
Biological Process
Cardiac atrium formation Source: BHF-UCL
Cardiac cell fate determination Source: BHF-UCL
Cardiac muscle cell differentiation Source: BHF-UCL
Cardiac vascular smooth muscle cell differentiation Source: BHF-UCL
Cardiac ventricle formation Source: BHF-UCL
Cardioblast anterior-lateral migration Source: BHF-UCL
Cardioblast migration to the midline involved in heart field formation Source: BHF-UCL
Embryonic heart tube morphogenesis Source: BHF-UCL
Endothelial cell differentiation Source: BHF-UCL
Gastrulation Source: BHF-UCL
Gene expression Source: Ensembl
Growth involved in heart morphogenesis Source: BHF-UCL
Heart looping Source: BHF-UCL
Heart morphogenesis Source: GO_Central
Lateral mesoderm development Source: BHF-UCL
Mesodermal cell migration Source: Ensembl
Mesoderm formation Source: GO_Central
Negative regulation of endodermal cell fate specification Source: BHF-UCL
Negative regulation of mesodermal cell fate specification Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: BHF-UCL
Neurogenesis Source: BHF-UCL
Notch signaling pathway Source: UniProtKB-KW
Positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Positive regulation of hepatocyte differentiation Source: BHF-UCL
Positive regulation of Notch signaling pathway Source: BHF-UCL
Positive regulation of Notch signaling pathway involved in heart induction Source: Ensembl
Positive regulation of striated muscle cell differentiation Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Secondary heart field specification Source: BHF-UCL
Signal transduction involved in regulation of gene expression Source: Ensembl
Sinoatrial node cell differentiation Source: BHF-UCL
Sinus venosus morphogenesis Source: BHF-UCL
Somite rostral/caudal axis specification Source: GO_Central
Cellular Location
Nucleus

Krup, A. L., Winchester, S. A., Ranade, S. S., Agrawal, A., Devine, W. P., Sinha, T., ... & Bruneau, B. G. (2023). A Mesp1-dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors. Development, 150(9), dev201229.

Ji, S., Xu, M., Cai, C., & He, X. (2023). MESP1-knockdown inhibits the proliferation and epithelial mesenchymal transition of hepatocellular carcinoma and enhances the tumor-suppressive effect of 5-fluorouracil. Biochemical and Biophysical Research Communications.

Lin, X., Swedlund, B., Ton, M. L. N., Ghazanfar, S., Guibentif, C., Paulissen, C., ... & Blanpain, C. (2022). Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors. Nature cell biology, 24(7), 1114-1128.

Liang, Q., Wang, S., Zhou, X., Li, Y., Xing, S., Yang, F., ... & Sun, N. (2022). Essential role of MESP1-RING1A complex in cardiac differentiation. Developmental Cell, 57(22), 2533-2549.

Ajima, R., Sakakibara, Y., Sakurai-Yamatani, N., Muraoka, M., & Saga, Y. (2021). Formal proof of the requirement of MESP1 and MESP2 in mesoderm specification and their transcriptional control via specific enhancers in mice. Development, 148(20), dev194613.

Haridhasapavalan, K. K., Ranjan, S. H., Bhattacharyya, S., & Thummer, R. P. (2021). Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor. Applied Microbiology and Biotechnology, 105, 2363-2376.

Wang, L., Zhang, F., Duan, F., Huang, R., Chen, X., Ming, J., & Na, J. (2020). Homozygous MESP1 knock-in reporter hESCs facilitated cardiovascular cell differentiation and myocardial infarction repair. Theranostics, 10(15), 6898.

Tandon, N., Goller, K., Wang, F., Soibam, B., Gagea, M., Jain, A. K., ... & Liu, Y. (2019). Aberrant expression of embryonic mesendoderm factor MESP1 promotes tumorigenesis. EBioMedicine, 50, 55-66.

Eskildsen, T. V., Ayoubi, S., Thomassen, M., Burton, M., Mandegar, M. A., Conklin, B. R., ... & Sheikh, S. P. (2019). MESP1 knock-down in human iPSC attenuates early vascular progenitor cell differentiation after completed primitive streak specification. Developmental biology, 445(1), 1-7.

Penaloza, J. S., Pappas, M. P., Hagen, H. R., Xie, N., & Chan, S. S. (2019). Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development. Biochemical and Biophysical Research Communications, 520(2), 284-290.

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For research use only. Not intended for any clinical use.

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