Mouse Anti-MFF Recombinant Antibody (CBFYM-2142) (CBMAB-M2324-FY)

Name: Mouse Anti-MFF Recombinant Antibody (CBFYM-2142)
SKU: CBMAB-M2324-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2142

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mitochondrial Fission Factor

Introduction

This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	56947
Mouse	75734
Rat	301563

UniProt ID

Human	Q9GZY8
Mouse	Q6PCP5
Rat	Q4KM98

Alternative Names

Mitochondrial Fission Factor; C2orf33; Chromosome 2 Open Reading Frame 33; EMPF2; GL004

Function

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

Biological Process

Mitochondrial fission Source: UniProtKB
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
Mitochondrial fusion Source: UniProtKB
Mitochondrion morphogenesis Source: MGI
Peroxisome fission Source: UniProtKB
Positive regulation of mitochondrial fission Source: UniProtKB
Positive regulation of protein targeting to membrane Source: UniProtKB
Positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
Protein targeting to mitochondrion Source: UniProtKB
Regulation of mitochondrion organization Source: UniProtKB
Regulation of peroxisome organization Source: UniProtKB
Release of cytochrome c from mitochondria Source: UniProtKB

Cellular Location

Peroxisome
Mitochondrion
Mitochondrion outer membrane
Other locations
synaptic vesicle

Involvement in disease

Encephalopathy due to defective mitochondrial and peroxisomal fission 2 (EMPF2):
An autosomal recessive disorder characterized by delayed psychomotor development, severe hypotonia with inability to walk, microcephaly, and abnormal signals in the basal ganglia. More variable features include early-onset seizures, optic atrophy, and peripheral neuropathy.

Topology

Cytoplasmic: 1-322
Helical: 323-340
Mitochondrial intermembrane: 341-342

Hua, R., Hai, Z., Gu, J., Guo, C., Xiao, Y., Zhao, P., ... & Wang, T. (2023). O-244 Mitochondrial fission factor (MFF) is required for female fertility and oocyte development. Human Reproduction, 38(Supplement_1), dead093-298.

Christen, M., Gutierrez‐Quintana, R., Vandenberghe, H., Kaczmarska, A., Penderis, J., José‐López, R., ... & Leeb, T. (2022). Mitochondrial fission factor (MFF) frameshift variant in Bullmastiffs with mitochondrial fission encephalopathy. Animal genetics, 53(6), 814-820.

Schrader, T. A., Carmichael, R. E., Islinger, M., Costello, J. L., Hacker, C., Bonekamp, N. A., ... & Schrader, M. (2022). PEX11β and FIS1 cooperate in peroxisome division independently of mitochondrial fission factor. Journal of Cell Science, 135(13), jcs259924.

Li, X., Wu, Q., Ma, F., Zhang, X., Cai, L., & Yang, X. (2022). Mitochondrial fission factor promotes cisplatin resistancein hepatocellular carcinoma: Mff promotes cisplatin resistance in HCC. Acta Biochimica et Biophysica Sinica, 54(3), 301.

Varuzhanyan, G., Chen, H., Rojansky, R., Ladinsky, M. S., McCaffery, J. M., & Chan, D. C. (2021). Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids. Biochimica et Biophysica Acta (BBA)-General Subjects, 1865(5), 129845.

Liu, Y., Gokhale, S., Jung, J., Zhu, S., Luo, C., Saha, D., ... & Xie, P. (2021). Mitochondrial fission factor is a novel interacting protein of the critical B cell survival regulator TRAF3 in B lymphocytes. Frontiers in Immunology, 12, 670338.

Passmore, J. B., Carmichael, R. E., Schrader, T. A., Godinho, L. F., Ferdinandusse, S., Lismont, C., ... & Schrader, M. (2020). Mitochondrial fission factor (MFF) is a critical regulator of peroxisome maturation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(7), 118709.

Sánchez-Alvarez, R., De Francesco, E. M., Fiorillo, M., Sotgia, F., & Lisanti, M. P. (2020). Mitochondrial fission factor (MFF) inhibits mitochondrial metabolism and reduces breast cancer stem cell (CSC) activity. Frontiers in Oncology, 10, 1776.

Lee, L., Seager, R., Nakamura, Y., Wilkinson, K. A., & Henley, J. M. (2019). Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff). PLoS One, 14(5), e0213116.

Seo, J. H., Agarwal, E., Chae, Y. C., Lee, Y. G., Garlick, D. S., Storaci, A. M., ... & Altieri, D. C. (2019). Mitochondrial fission factor is a novel Myc-dependent regulator of mitochondrial permeability in cancer. EBioMedicine, 48, 353-363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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