Mouse Anti-MFNG (AA 214-291) Recombinant Antibody (CBFYM-2158) (CBMAB-M2340-FY)

Name: Mouse Anti-MFNG (AA 214-291) Recombinant Antibody (CBFYM-2158)
SKU: CBMAB-M2340-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2158

Application

S-ELISA, ELISA, WB-Re

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 214-291

More Infomation

Target

Full Name

MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Introduction

This gene is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling.

Entrez Gene ID

4242

UniProt ID

O00587

Alternative Names

MFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase; EC 2.4.1.222; O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase; Manic Fringe (Drosophila) Homolog; Manic Fringe Homolog (Drosophila)

Function

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules (PubMed:10935626).

Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).

Biological Process

Blastocyst formation Source: Ensembl
Marginal zone B cell differentiation Source: UniProtKB
Pattern specification process Source: ProtInc
Positive regulation of Notch signaling pathway Source: Ensembl
Positive regulation of protein binding Source: Ensembl
Regulation of Notch signaling pathway Source: UniProtKB

Cellular Location

Golgi apparatus membrane

Topology

Cytoplasmic: 1-7
Helical: 8-27
Lumenal: 28-321

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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