Mouse Anti-MGAT1 Recombinant Antibody (CBFYM-2166) (CBMAB-M2348-FY)

This product is mouse antibody that recognizes MGAT1. The antibody CBFYM-2166 can be used for immunoassay techniques such as: ELISA.
See all MGAT1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2166

Antibody Isotype

IgM, k

Application

ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgM, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Antiserum

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MANNOSYL (ALPHA-1,3-)-GLYCOPROTEIN BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE

Introduction

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

Entrez Gene ID

4245

UniProt ID

P26572

Alternative Names

Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase; N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase I; Alpha-1,3-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase; EC 2.4.1.101; GlcNAc-T I; GLYT1; GLCT1

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Biological Process

In utero embryonic development Source: Ensembl
Protein glycosylation Source: GO_Central
Protein N-linked glycosylation Source: GO_Central
Protein N-linked glycosylation via asparagine Source: UniProtKB
UDP-N-acetylglucosamine catabolic process Source: Ensembl
Viral protein processing Source: Reactome

Cellular Location

Golgi apparatus
Golgi apparatus membrane
Other locations
perinuclear region
Note: Co-localizes with BRI3 isoform 1 at the perinuclear region.

Topology

Cytoplasmic: 1-6
Helical: 7-29
Lumenal: 30-445

References

Bi, W., Yang, M., Xing, P., & Huang, T. (2022). MicroRNA miR-331-3p suppresses osteosarcoma progression via the Bcl-2/Bax and Wnt/β-Catenin signaling pathways and the epithelial-mesenchymal transition by targeting N-acetylglucosaminyltransferase I (MGAT1). Bioengineered, 13(6), 14159-14174.

Zhu, Q., Zhan, D., Yang, Y., Chong, Y., Xue, H., & Zhu, P. (2022). LINC00173 promotes Wilms’ tumor progression through MGAT1-mediated MUC3A N-glycosylation. Cell Cycle, 21(17), 1795-1810.

Liu, W., Li, X., Tan, X., Huang, X., & Tian, B. (2021). MicroRNA-204-3p inhibits metastasis of pancreatic cancer via downregulating MGAT1. J BUON, 26(5), 2149-2156.

Biswas, B., Batista, F., Akintayo, A., Aguilan, J., & Stanley, P. (2020). Transgenic rescue of spermatogenesis in males with Mgat1 deleted in germ cells. Frontiers in cell and developmental biology, 8, 212.

Chen, P., Zhou, C., Li, B., & Yang, C. (2020). Circular RNA MGAT1 regulates cell proliferation and apoptosis in hypoxia-induced cardiomyocytes through miR-34a/YAP1 axis. International Journal of Clinical and Experimental Pathology, 13(10), 2474.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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