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Mouse Anti-MGMT Recombinant Antibody (CBFYM-0636) (CBMAB-M0771-FY)

This product is mouse antibody that recognizes MGMT. The antibody CBFYM-0636 can be used for immunoassay techniques such as: FC, IHC, WB.
See all MGMT antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0636
Antibody Isotype
IgG2b
Application
FC, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
O-6-Methylguanine-DNA Methyltransferase
Introduction
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Entrez Gene ID
4255
UniProt ID
P16455
Alternative Names
O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; -O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase
Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Biological Process
Cellular response to ionizing radiation Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Cellular response to oxidative stress Source: Ensembl
DNA dealkylation involved in DNA repair Source: Ensembl
DNA ligation Source: ProtInc
DNA repair Source: GO_Central
Mammary gland epithelial cell differentiation Source: Ensembl
Negative regulation of apoptotic process Source: BHF-UCL
Positive regulation of double-strand break repair Source: BHF-UCL
Regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Response to ethanol Source: Ensembl
Response to folic acid Source: Ensembl
Response to toxic substance Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl
Cellular Location
Nucleus

Chen, Y., Qu, W., Tu, J., & Qi, H. (2023). Implications of Advances in Studies of O6-Methylguanine-DNA-Methyltransferase for Tumor Prognosis and Treatment. Frontiers in Bioscience-Landmark, 28(9), 197.

Chen, X., Sun, J., Li, Y., Jiang, W., Li, Z., Mao, J., ... & Tan, G. (2023). Proteomic and metabolomic analyses illustrate the mechanisms of expression of the O6‐methylguanine‐DNA methyltransferase gene in glioblastoma. CNS Neuroscience & Therapeutics.

Kostka, T., Empl, M. T., Seiwert, N., Geisen, S. M., Hoffmann, P., Adam, J., ... & Steinberg, P. (2021). Repair of O 6-carboxymethylguanine adducts by O 6-methylguanine-DNA methyltransferase in human colon epithelial cells. Carcinogenesis, 42(8), 1110-1118.

McAleenan, A., Kelly, C., Spiga, F., Kernohan, A., Cheng, H. Y., Dawson, S., ... & Kurian, K. M. (2021). Prognostic value of test (s) for O6‐methylguanine–DNA methyltransferase (MGMT) promoter methylation for predicting overall survival in people with glioblastoma treated with temozolomide. Cochrane Database of Systematic Reviews, (3).

Yu, W., Zhang, L., Wei, Q., & Shao, A. (2020). O6-methylguanine-DNA methyltransferase (MGMT): challenges and new opportunities in glioma chemotherapy. Frontiers in oncology, 9, 1547.

Dovek, L., Nguyen, N. T., Ozer, B. H., Li, N., Elashoff, R. M., Green, R. M., ... & Lai, A. (2019). Correlation of commercially available quantitative MGMT (O-6-methylguanine-DNA methyltransferase) promoter methylation scores and GBM patient survival. Neuro-Oncology Practice, 6(3), 194-202.

Feldheim, J., Kessler, A. F., Monoranu, C. M., Ernestus, R. I., Löhr, M., & Hagemann, C. (2019). Changes of O6-methylguanine DNA methyltransferase (MGMT) promoter methylation in glioblastoma relapse—a meta-analysis type literature review. Cancers, 11(12), 1837.

Yang, L., Li, W., Zhao, Y., Zhong, S., Wang, X., Jiang, S., ... & Zhao, G. (2019). Computational study of novel natural inhibitors targeting O6-methylguanine-DNA methyltransferase. World neurosurgery, 130, e294-e306.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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