Rabbit Anti-MICA Recombinant Antibody (
9F1) (V2LY-0725-LY1263)

Name: Rabbit Anti-MICA Recombinant Antibody (9F1)
SKU: V2LY-0725-LY1263
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-MICA Recombinant Antibody (9F1) (V2LY-0725-LY1263) in IHC

IHC image of V2LY-0725-LY1263 diluted at 1:80 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.

Rabbit Anti-MICA Recombinant Antibody (9F1) (V2LY-0725-LY1263) in IHC

IHC image of V2LY-0725-LY1263 diluted at 1:80 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9F1

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from Human MICA.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MHC Class I Polypeptide-Related Sequence A

Entrez Gene ID

100507436

UniProt ID

Q29983

Function

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Defense response to bacterium Source: UniProtKB
Defense response to virus Source: UniProtKB
Gamma-delta T cell activation Source: UniProtKB
Immune response to tumor cell Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of natural killer cell activation Source: CACAO
Negative regulation of natural killer cell mediated cytotoxicity Source: CACAO
Response to heat Source: UniProtKB
T cell mediated cytotoxicity Source: UniProtKB

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane
Note: Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.

Involvement in disease

Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
Psoriasis 1 (PSORS1):
A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Psoriatic arthritis (PSORAS):
An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Topology

Extracellular: 24-307
Helical: 308-328
Cytoplasmic: 329-383

PTM

N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.

Ren, X., Mao, A., Tan, S., Liu, J., & Wei, X. (2022). Analysis of the association between MICA gene polymorphisms and schizophrenia. Journal of Clinical Laboratory Analysis, 36(11), e24721.

Espinoza, I., Agarwal, S., Sakiyama, M., Shenoy, V., Orr, W. S., Al Diffalha, S., ... & Gomez, C. R. (2021). Expression of MHC class I polypeptide-related sequence A (MICA) in colorectal cancer.

Closa, L., Vidal, F., Herrero, M. J., & Caro, J. L. (2021). High‐throughput genotyping of HLA‐G, HLA‐F, MICA, and MICB and analysis of frequency distributions in healthy blood donors from Catalonia. HLA, 97(5), 420-427.

Otoyama, Y., Arai, J., Goto, K., Nozawa, H., Nakagawa, R., Muroyama, R., ... & Yoshida, H. (2021). Retinoids decrease soluble MICA concentration by inhibiting the enzymatic activity of ADAM9 and ADAM10. Anticancer Research, 41(5), 2307-2320.

Gong, X., Chen, P., Ma, P., Gao, J., Yang, J., Guo, H., ... & He, Y. (2020). MICA polymorphisms associated with antithyroid drug‐induced agranulocytosis in the Chinese Han population. Immunity, Inflammation and Disease, 8(4), 695-703.

Plasil, M., Wijkmark, S., Elbers, J. P., Oppelt, J., Burger, P. A., & Horin, P. (2019). The major histocompatibility complex of Old World camelids: class I and class I‐related genes. Hla, 93(4), 203-215.

Sakiyama, M. J., Espinoza, I., Reddy, A., de Carlo, F., Kumar, A., Levenson, A. S., ... & Gomez, C. R. (2019). Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer. Experimental and molecular pathology, 108, 173-182.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MICA Matched Antibody Pair (735) (CAT#: APMAB-735LY)

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Isotype control

For research use only. Not intended for any clinical use.

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