Mouse Anti-MICAL3 (AA 251-340) Recombinant Antibody (CBFYM-2214) (CBMAB-M2397-FY)

Name: Mouse Anti-MICAL3 (AA 251-340) Recombinant Antibody (CBFYM-2214)
SKU: CBMAB-M2397-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2214

Application

ELISA, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: DDQHWSDSPS DADRELRLPC PAEGEAELEL RVSEDEEKLP ASPKHQERGP SQATSPIRSP QESALLFIPV HSPSTEGPQL PPVPAATQEK

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 251-340

More Infomation

Target

Full Name

microtubule associated monoxygenase, calponin and LIM domain containing 3

Introduction

MICAL3 is a Protein Coding gene. Gene Ontology annotations related to this gene include actin binding and Rab GTPase binding. An important paralog of this gene is MICAL2.

Entrez Gene ID

57553

UniProt ID

Q7RTP6

Alternative Names

Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 3; Molecule Interacting With CasL Protein 3; MICAL-3; [F-Actin]-Methionine Sulfoxide Oxidase MICAL3; Protein-Methionine Sulfoxide Oxidase MICAL3; Flavoprotein Oxidoreductase MICAL3

Function

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H2O2. Seems to act as Rab effector protein and plays a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.

Biological Process

Actin filament depolymerization Source: UniProtKB
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cytoskeleton organization Source: UniProtKB
Exocytosis Source: UniProtKB-KW

Cellular Location

Nucleus
Cytoskeleton
spindle
cilium basal body
Cytoplasm 1 Publication
Other locations
cell cortex
Midbody
Note: Mainly localizes in the nucleus.

Kim, J., Lee, H., Roh, Y. J., Kim, H. U., Shin, D., Kim, S., ... & Lee, B. C. (2020). Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3. IUCrJ, 7(1), 90-99.

Tominaga, K., Minato, H., Murayama, T., Sasahara, A., Nishimura, T., Kiyokawa, E., ... & Gotoh, N. (2019). Semaphorin signaling via MICAL3 induces symmetric cell division to expand breast cancer stem-like cells. Proceedings of the National Academy of Sciences, 116(2), 625-630.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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