Mouse Anti-MID1IP1 Recombinant Antibody (8G8) (CBMAB-A5484-LY)

The product is antibody recognizes MID1IP1. The antibody 8G8 immunoassay techniques such as: WB, ELISA.
See all MID1IP1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

8G8

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

MID1IP1 (NP_067065.1, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))

Entrez Gene ID

58526

UniProt ID

Q9NPA3

Alternative Names

FLJ10386; G12-like; MIG12; STRAIT11499; THRSPL

Function

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).

Biological Process

Lipid metabolic process Source: UniProtKB-KW
Negative regulation of microtubule depolymerization Source: UniProtKB
Positive regulation of fatty acid biosynthetic process Source: UniProtKB
Positive regulation of ligase activity Source: UniProtKB
Protein polymerization Source: UniProtKB
Regulation of lipid biosynthetic process Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Cytoskeleton
Note: Associated with microtubules.

References

Suh, J. Y., Lee, H. J., Sim, D. Y., Park, J. E., Ahn, C. H., Park, S. Y., ... & Kim, S. H. (2023). Hypolipogenic effects of Icariside E4 via phosphorylation of AMPK and inhibition of MID1IP1 in HepG2 cells. Phytotherapy Research, 37(1), 7-14.

Jung, J. H., Lee, D., Ko, H. M., & Jang, H. J. (2021). Inhibition of CNOT2 induces apoptosis via MID1IP1 in colorectal cancer cells by activating p53. Biomolecules, 11(10), 1492.

Jung, J. H., Lee, H. J., Kim, J. H., Sim, D. Y., Im, E., Kim, S., ... & Kim, S. H. (2020). Colocalization of MID1IP1 and c-Myc is critically involved in liver cancer growth via regulation of ribosomal protein L5 and L11 and CNOT2. Cells, 9(4), 985.

Meng, X., Liao, Z., Wei, Y., Sun, B., Jia, L., Liang, M., & Xu, H. (2020). Cloning and characterization of MID1 interacting protein 1 (mid1ip1) from tiger puffer (Takifugu rubripes), and its gene expression in response to dietary bile acid and lipid levels. Aquaculture Reports, 17, 100363.

Kim, M. J., Sim, D. Y., Lee, H. M., Lee, H. J., & Kim, S. H. (2019). Hypolipogenic effect of shikimic acid via inhibition of MID1IP1 and phosphorylation of AMPK/ACC. International journal of molecular sciences, 20(3), 582.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Mouse Anti-MID1IP1 (AA 1-183) Recombinant Antibody (CBFYM-2222)

Mouse Anti-MID1IP1 Recombinant Antibody (CBFYM-2220)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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