Human Recombinant MID1IP1 protein, His Tag (V2LY-0526-LY5584)

Name: Human Recombinant MID1IP1 protein, His Tag
SKU: V2LY-0526-LY5584
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MID1IP1 protein, His Tag consist of Amino Acid: 1-183 and predicts a molecular mass of 22 kDa.

Molecule Mass

22 kDa

Sequence

Amino Acid: 1-183

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))

Function

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).

Biological Process

Lipid metabolic process Source: UniProtKB-KW
Negative regulation of microtubule depolymerization Source: UniProtKB
Positive regulation of fatty acid biosynthetic process Source: UniProtKB
Positive regulation of ligase activity Source: UniProtKB
Protein polymerization Source: UniProtKB
Regulation of lipid biosynthetic process Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Cytoskeleton
Note: Associated with microtubules.

Suh, J. Y., Lee, H. J., Sim, D. Y., Park, J. E., Ahn, C. H., Park, S. Y., ... & Kim, S. H. (2023). Hypolipogenic effects of Icariside E4 via phosphorylation of AMPK and inhibition of MID1IP1 in HepG2 cells. Phytotherapy Research, 37(1), 7-14.

Jung, J. H., Lee, D., Ko, H. M., & Jang, H. J. (2021). Inhibition of CNOT2 induces apoptosis via MID1IP1 in colorectal cancer cells by activating p53. Biomolecules, 11(10), 1492.

Jung, J. H., Lee, H. J., Kim, J. H., Sim, D. Y., Im, E., Kim, S., ... & Kim, S. H. (2020). Colocalization of MID1IP1 and c-Myc is critically involved in liver cancer growth via regulation of ribosomal protein L5 and L11 and CNOT2. Cells, 9(4), 985.

Meng, X., Liao, Z., Wei, Y., Sun, B., Jia, L., Liang, M., & Xu, H. (2020). Cloning and characterization of MID1 interacting protein 1 (mid1ip1) from tiger puffer (Takifugu rubripes), and its gene expression in response to dietary bile acid and lipid levels. Aquaculture Reports, 17, 100363.

Kim, M. J., Sim, D. Y., Lee, H. M., Lee, H. J., & Kim, S. H. (2019). Hypolipogenic effect of shikimic acid via inhibition of MID1IP1 and phosphorylation of AMPK/ACC. International journal of molecular sciences, 20(3), 582.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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