Mouse Anti-MITF Recombinant Antibody (CBT4499) (V2LY-0625-LY573)

Name: Mouse Anti-MITF Recombinant Antibody (CBT4499)
SKU: V2LY-0625-LY573
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MITF Recombinant Antibody (CBT4499) (V2LY-0625-LY573) in ELISA

Mouse Anti-MITF Recombinant Antibody (CBT4499) (V2LY-0625-LY573) in WB

Mouse Anti-MITF Recombinant Antibody (CBT4499) (V2LY-0625-LY573) in FC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4499

Application

Immunogen

Purified recombinant fragment of human MITF (AA: 1-114) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Melanogenesis Associated Transcription Factor

Entrez Gene ID

4286

UniProt ID

O75030

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Biological Process

Bone remodeling Source: Ensembl
Camera-type eye development Source: Ensembl
Canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: Ensembl
Cell fate commitment Source: Ensembl
Melanocyte differentiation Source: GO_Central
Negative regulation of cell migration Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Osteoclast differentiation Source: Ensembl
Positive regulation of DNA-templated transcription, initiation Source: CACAO
Positive regulation of gene expression Source: CACAO
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Protein-containing complex assembly Source: UniProtKB
Regulation of cell population proliferation Source: Ensembl
Regulation of osteoclast differentiation Source: Ensembl
Regulation of RNA biosynthetic process Source: CACAO
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Cytoplasm 1 Publication
Nucleus
Note: Found exclusively in the nucleus upon phosphorylation.

Involvement in disease

Waardenburg syndrome 2A (WS2A):
WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Tietz albinism-deafness syndrome (TADS):
An autosomal dominant disorder characterized by generalized hypopigmentation and congenital, bilateral, profound sensorineural deafness.
Melanoma, cutaneous malignant 8 (CMM8):
A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Coloboma, osteopetrosis, microphthalmia, macrocephaly, albinism, and deafness (COMMAD):
An autosomal recessive syndrome characterized by severe microphthalmia, profound congenital sensorineural hearing loss, lack of pigment in the hair, skin, and eyes, macrocephaly, facial dysmorphism, and osteopetrosis.

PTM

Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (PubMed:10587587). Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (PubMed:10673502). Phosphorylated in response to blue light (415nm) (PubMed:28842328).
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.

Gelmi, M. C., Houtzagers, L. E., Strub, T., Krossa, I., & Jager, M. J. (2022). Mitf in normal melanocytes, cutaneous and uveal melanoma: a delicate balance. International journal of molecular sciences, 23(11), 6001.

Truong, X. T., Lee, Y. S., Nguyen, T. T., Kim, H. J., Kim, S. H., Moon, C., ... & Jeon, T. I. (2022). SMILE Downregulation during Melanogenesis Induces MITF Transcription in B16F10 Cells. International Journal of Molecular Sciences, 23(23), 15094.

Abrahamian, C., & Grimm, C. (2021). Endolysosomal cation channels and MITF in melanocytes and melanoma. Biomolecules, 11(7), 1021.

Yun, C. Y., Roh, E., Kim, S. H., Han, J., Lee, J., Jung, D. E., ... & Kim, Y. (2020). Stem cell factor-inducible MITF-M expression in therapeutics for acquired skin hyperpigmentation. Theranostics, 10(1), 340.

Lv, J., Fu, Y., Cao, Y., Jiang, S., Yang, Y., Song, G., ... & Gao, R. (2020). Isoliquiritigenin inhibits melanogenesis, melanocyte dendricity and melanosome transport by regulating ERK‐mediated MITF degradation. Experimental Dermatology, 29(2), 149-157.

Kim, J. H., Hong, A. R., Kim, Y. H., Yoo, H., Kang, S. W., Chang, S. E., & Song, Y. (2020). JNK suppresses melanogenesis by interfering with CREB-regulated transcription coactivator 3-dependent MITF expression. Theranostics, 10(9), 4017.

Flesher, J. L., Paterson‐Coleman, E. K., Vasudeva, P., Ruiz‐Vega, R., Marshall, M., Pearlman, E., ... & Ganesan, A. K. (2020). Delineating the role of MITF isoforms in pigmentation and tissue homeostasis. Pigment cell & melanoma research, 33(2), 279-292.

Raja, D. A., Gotherwal, V., Burse, S. A., Subramaniam, Y. J., Sultan, F., Vats, A., ... & Natarajan, V. T. (2020). pH‐controlled histone acetylation amplifies melanocyte differentiation downstream of MITF. EMBO reports, 21(1), e48333.

Basu, R., Kulkarni, P., Qian, Y., Walsh, C., Arora, P., Davis, E., ... & Kopchick, J. J. (2019). Growth hormone upregulates melanocyte-inducing transcription factor expression and activity via JAK2-STAT5 and SRC signaling in GH receptor-positive human melanoma. Cancers, 11(9), 1352.

Goding, C. R., & Arnheiter, H. (2019). MITF—the first 25 years. Genes & development, 33(15-16), 983-1007.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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