Mouse Anti-MKNK2 Recombinant Antibody (2A10) (CBMAB-A5503-LY)

Name: Mouse Anti-MKNK2 Recombinant Antibody (2A10)
SKU: CBMAB-A5503-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A10

Application

WB, ELISA

Immunogen

MKNK2 (AAH18345, 1 a.a. ~ 158 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MAP kinase interacting serine/threonine kinase 2

Entrez Gene ID

2872

UniProt ID

Q9HBH9

Alternative Names

GPRK7; MNK2

Function

Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As2O3-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Cellular response to arsenic-containing substance Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
Hemopoiesis Source: UniProtKB
Intracellular signal transduction Source: UniProtKB
Peptidyl-serine phosphorylation Source: GO_Central
Protein autophosphorylation Source: GO_Central
Protein phosphorylation Source: UniProtKB
Regulation of translation Source: UniProtKB-KW

Cellular Location

Isoform 2:
Nucleus
PML body
Isoform 1:
Cytoplasm

PTM

Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As2O3 treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A.

Aijaz, A., Padilla, I., & Smith, K. (2023). Activity of MKNK2 Isoforms Studied by Phosphorylation with p38a-MAPK.

Kuang, P., Zhang, J., Wan, F., Zhang, Y., Zhang, W., & Zhang, X. (2023, May). MKNK2 as A Prognosis-Related Biomarker for Gastric Cancer. In Proceedings of the 2023 15th International Conference on Bioinformatics and Biomedical Technology (pp. 61-68).

Vaughan, N., Collier, A., Harrell, J., & Clark, A. (2023). Expression of MKNK2 Isoforms in Cancer Cell Lines.

Stefanie, A., & Diaz, M. (2023). Endogenous levels of MKNK2 Isoforms in Normal and Cancer Cell Lines.

Phan, V., & Silva, A. (2023). Comparison of MKNK2 Isoform Levels in Normal and Cancer Cells.

Huang, B., Guo, S., Zhang, Y., Lin, P., Lin, C., Chen, M., ... & Wen, Z. (2022). MiR‐223‐3p alleviates trigeminal neuropathic pain in the male mouse by targeting MKNK2 and MAPK/ERK signaling. Brain and Behavior, 12(7), e2634.

Wang, J., Da, C., Su, Y., Song, R., & Bai, Z. (2021). MKNK2 enhances chemoresistance of ovarian cancer by suppressing autophagy via miR-125b. Biochemical and Biophysical Research Communications, 556, 31-38.

Liu, H., Gong, Z., Li, K., Zhang, Q., Xu, Z., & Xu, Y. (2021). SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma. Journal of Experimental & Clinical Cancer Research, 40(1), 1-16.

Qin, M., Liang, Z., Qin, H., Huo, Y., Wu, Q., Yang, H., & Tang, G. (2021). Novel prognostic biomarkers in gastric cancer: CGB5, MKNK2, and PAPPA2. Frontiers in Oncology, 11, 683582.

Liu, B., Sun, Y., Tang, M., Liang, C., Huang, C. P., Niu, Y., ... & Chang, C. (2020). The miR-361-3p increases enzalutamide (Enz) sensitivity via targeting the ARv7 and MKNK2 to better suppress the Enz-resistant prostate cancer. Cell Death & Disease, 11(9), 807.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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