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Mouse Anti-MKRN3 (AA 437-507) Recombinant Antibody (CBFYM-2268) (CBMAB-M2453-FY)

This product is mouse antibody that recognizes MKRN3. The antibody CBFYM-2268 can be used for immunoassay techniques such as: ELISA, ICC, IF.
See all MKRN3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2268
Antibody Isotype
IgG2b, k
Application
ELISA, ICC, IF

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, k
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 437-507

Target

Full Name
makorin ring finger protein 3
Introduction
The protein encoded by this gene contains a RING zinc finger motif and several C3H zinc finger motifs. This gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping this gene.
Entrez Gene ID
7681
UniProt ID
Q13064
Alternative Names
Makorin Ring Finger Protein 3; Zinc Finger Protein 127; RING-Type E3 Ubiquitin Transferase Makorin-3; RING Finger Protein 63; ZNF127; D15S9
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Biological Process
Protein polyubiquitination Source: FlyBase
Protein ubiquitination Source: GO_Central
Cellular Location
Ribonucleoprotein complex
Involvement in disease
Precocious puberty, central 2 (CPPB2):
A condition defined as the development of secondary sexual characteristics in boys and girls at a chronological age that is 2.5 standard deviations below the mean age at onset of puberty in the population. Central precocious puberty results from premature activation of the hypothalamic-pituitary-gonadal axis.

Palumbo, S., Cirillo, G., Aiello, F., Papparella, A., Miraglia del Giudice, E., & Grandone, A. (2022). MKRN3 role in regulating pubertal onset: the state of art of functional studies. Frontiers in Endocrinology, 13, 991322.

Seraphim, C. E., Canton, A. P. M., Montenegro, L., Piovesan, M. R., Macedo, D. B., Cunha, M., ... & Latronico, A. C. (2021). Genotype–phenotype correlations in central precocious puberty caused by MKRN3 mutations. The Journal of Clinical Endocrinology & Metabolism, 106(4), e1041-e1050.

Li, M., Chen, Y., Liao, B., Tang, J., Zhong, J., & Lan, D. (2021). The role of kisspeptin and MKRN3 in the diagnosis of central precocious puberty in girls. Endocrine connections, 10(9), 1147-1154.

Li, K., Zheng, X., Tang, H., Zang, Y. S., Zeng, C., Liu, X., ... & Wang, Y. (2021). E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non–small cell lung cancer. Journal of Experimental Medicine, 218(8), e20210151.

Li, C., Han, T., Li, Q., Zhang, M., Guo, R., Yang, Y., ... & Hu, R. (2021). MKRN3-mediated ubiquitination of Poly (A)-binding proteins modulates the stability and translation of GNRH1 mRNA in mammalian puberty. Nucleic Acids Research, 49(7), 3796-3813.

Abreu, A. P., Toro, C. A., Song, Y. B., Navarro, V. M., Bosch, M. A., Eren, A., ... & Kaiser, U. B. (2020). MKRN3 inhibits the reproductive axis through actions in kisspeptin-expressing neurons. The Journal of Clinical Investigation, 130(8), 4486-4500.

Li, C., Lu, W., Yang, L., Li, Z., Zhou, X., Guo, R., ... & Hu, R. (2020). MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3. National Science Review, 7(3), 671-685.

Naulé, L., Abreu, A., Kaiser, U., Plant, T., Avendaño, M., Vazquez, M., ... & Galiana-Arnoux, D. (2019, July). Evolutionary conservation of MKRN3 and other makorins and their roles in puberty initiation and endocrine functions. In Seminars in reproductive medicine (Vol. 37, No. 04, pp. 166-173). 333 Seventh Avenue, New York, NY 10001, USA.: Thieme Medical Publishers.

Valadares, L. P., Meireles, C. G., De Toledo, I. P., Santarem de Oliveira, R., Gonçalves de Castro, L. C., Abreu, A. P., ... & Lofrano-Porto, A. (2019). MKRN3 mutations in central precocious puberty: a systematic review and meta-analysis. Journal of the Endocrine Society, 3(5), 979-995.

Yellapragada, V., Liu, X., Lund, C., Känsäkoski, J., Pulli, K., Vuoristo, S., ... & Raivio, T. (2019). MKRN3 interacts with several proteins implicated in puberty timing but does not influence GNRH1 expression. Frontiers in endocrinology, 10, 48.

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For research use only. Not intended for any clinical use.

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