Human MKRN3 ELISA Kit (V2LY-0626-LY5450)

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Tested Data
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Basic Information

Sensitivity
0.019 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
55.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
makorin ring finger protein 3
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Biological Process
Protein polyubiquitination Source: FlyBase
Protein ubiquitination Source: GO_Central
Cellular Location
Ribonucleoprotein complex
Involvement in disease
Precocious puberty, central 2 (CPPB2):
A condition defined as the development of secondary sexual characteristics in boys and girls at a chronological age that is 2.5 standard deviations below the mean age at onset of puberty in the population. Central precocious puberty results from premature activation of the hypothalamic-pituitary-gonadal axis.

Palumbo, S., Cirillo, G., Aiello, F., Papparella, A., Miraglia del Giudice, E., & Grandone, A. (2022). MKRN3 role in regulating pubertal onset: the state of art of functional studies. Frontiers in Endocrinology, 13, 991322.

Seraphim, C. E., Canton, A. P. M., Montenegro, L., Piovesan, M. R., Macedo, D. B., Cunha, M., ... & Latronico, A. C. (2021). Genotype–phenotype correlations in central precocious puberty caused by MKRN3 mutations. The Journal of Clinical Endocrinology & Metabolism, 106(4), e1041-e1050.

Li, M., Chen, Y., Liao, B., Tang, J., Zhong, J., & Lan, D. (2021). The role of kisspeptin and MKRN3 in the diagnosis of central precocious puberty in girls. Endocrine connections, 10(9), 1147-1154.

Li, K., Zheng, X., Tang, H., Zang, Y. S., Zeng, C., Liu, X., ... & Wang, Y. (2021). E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non–small cell lung cancer. Journal of Experimental Medicine, 218(8), e20210151.

Li, C., Han, T., Li, Q., Zhang, M., Guo, R., Yang, Y., ... & Hu, R. (2021). MKRN3-mediated ubiquitination of Poly (A)-binding proteins modulates the stability and translation of GNRH1 mRNA in mammalian puberty. Nucleic Acids Research, 49(7), 3796-3813.

Abreu, A. P., Toro, C. A., Song, Y. B., Navarro, V. M., Bosch, M. A., Eren, A., ... & Kaiser, U. B. (2020). MKRN3 inhibits the reproductive axis through actions in kisspeptin-expressing neurons. The Journal of Clinical Investigation, 130(8), 4486-4500.

Li, C., Lu, W., Yang, L., Li, Z., Zhou, X., Guo, R., ... & Hu, R. (2020). MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3. National Science Review, 7(3), 671-685.

Naulé, L., Abreu, A., Kaiser, U., Plant, T., Avendaño, M., Vazquez, M., ... & Galiana-Arnoux, D. (2019, July). Evolutionary conservation of MKRN3 and other makorins and their roles in puberty initiation and endocrine functions. In Seminars in reproductive medicine (Vol. 37, No. 04, pp. 166-173). 333 Seventh Avenue, New York, NY 10001, USA.: Thieme Medical Publishers.

Valadares, L. P., Meireles, C. G., De Toledo, I. P., Santarem de Oliveira, R., Gonçalves de Castro, L. C., Abreu, A. P., ... & Lofrano-Porto, A. (2019). MKRN3 mutations in central precocious puberty: a systematic review and meta-analysis. Journal of the Endocrine Society, 3(5), 979-995.

Yellapragada, V., Liu, X., Lund, C., Känsäkoski, J., Pulli, K., Vuoristo, S., ... & Raivio, T. (2019). MKRN3 interacts with several proteins implicated in puberty timing but does not influence GNRH1 expression. Frontiers in endocrinology, 10, 48.

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For research use only. Not intended for any clinical use.

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