Rabbit Anti-MLKL Recombinant Antibody (A1129) (CBMAB-AP11889LY)

Name: Rabbit Anti-MLKL Recombinant Antibody (A1129)
SKU: CBMAB-AP11889LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

A1129

Application

IHC, WB

Immunogen

Recombinant protein of human MLKL

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Mixed Lineage Kinase Domain Like Pseudokinase

Introduction

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Entrez Gene ID

197259

UniProt ID

Q8NB16

Alternative Names

Mixed Lineage Kinase Domain Like Pseudokinase; HMLKL; Mixed Lineage Kinase Domain-Like Protein; Mixed Lineage Kinase Domain-Like;

Function

A11458

Biological Process

Cell surface receptor signaling pathway Source: InterPro
Defense response to virus Source: UniProtKB
Execution phase of necroptosis Source: UniProtKB
Necroptotic process Source: UniProtKB
Necroptotic signaling pathway Source: UniProtKB
Protein homotrimerization Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Plasma membrane
Cell membrane
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity).

PTM

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:22265413). It also induces homotrimerization and localization to the plasma membrane (PubMed:22265413).

Yang, Y., Wang, L., Zhang, H., & Luo, L. (2022). Mixed lineage kinase domain-like pseudokinase-mediated necroptosis aggravates periodontitis progression. Journal of Molecular Medicine, 1-10.

Kamal, A. M., Sebak, S. A., & Sanad, E. F. (2021). Mixed Lineage Kinase Domain-Like Pseudokinase (MLKL) Gene Expression in Human Atherosclerosis with and without Type 2 Diabetes Mellitus. Iranian Biomedical Journal, 25(4), 265.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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