Mouse Anti-MPST (AA 1-100) Recombinant Antibody (CBFYM-2520) (CBMAB-M2707-FY)

Name: Mouse Anti-MPST (AA 1-100) Recombinant Antibody (CBFYM-2520)
SKU: CBMAB-M2707-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2520

Application

ELISA, IP

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MASPQLCRAL VSAQWVAEAL RAPRAGQPLQ LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDQCSDRTS PYDHMLPGAE HFAEYAGRLG VGAATHVVIY

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-100

More Infomation

Target

Full Name

mercaptopyruvate sulfurtransferase

Introduction

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein, which appears to be cytoplasmic, and thiosulfate sulfurtransferase, which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria. Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.

Entrez Gene ID

4357

UniProt ID

P25325

Alternative Names

Mercaptopyruvate Sulfurtransferase; TRNA Thiouridin Modification Protein 1; Human Liver Rhodanese; TST2; MST; 3-Mercaptopyruvate Sulfurtransferase; Testicular Tissue Protein Li 200; EC 2.8.1.2; TUM1

Function

Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H2S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.

Biological Process

Cyanate catabolic process Source: ProtInc
Hydrogen sulfide biosynthetic process Source: UniProtKB
Kidney development Source: Ensembl
Liver development Source: Ensembl
Response to toxic substance Source: ProtInc
Spinal cord development Source: Ensembl
Sulfur amino acid catabolic process Source: Reactome
Transsulfuration Source: GO_Central

Cellular Location

Mitochondrion
Cytoplasm By similarity
Other locations
synaptosome

Involvement in disease

Aberrant MPST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine (PubMed:4973015, PubMed:4690911 and PubMed:6945862). Some cases have associated mental retardation (PubMed:4973015 and PubMed:6945862).

Pedre, B., Talwar, D., Barayeu, U., Schilling, D., Luzarowski, M., Sokolowski, M., ... & Dick, T. P. (2023). 3-Mercaptopyruvate sulfur transferase is a protein persulfidase. Nature Chemical Biology, 19(4), 507-517.

Nagahara, N., & Ito, T. (2023). 3-Mercaptopyruvate sulfurtransferase: the molecular and functional properties. In Sulfurtransferases (pp. 1-53). Academic Press.

Pedre, B., & Dick, T. P. (2021). 3-Mercaptopyruvate sulfurtransferase: an enzyme at the crossroads of sulfane sulfur trafficking. Biological chemistry, 402(3), 223-237.

Augsburger, F., Randi, E. B., Jendly, M., Ascencao, K., Dilek, N., & Szabo, C. (2020). Role of 3-mercaptopyruvate sulfurtransferase in the regulation of proliferation, migration, and bioenergetics in murine colon cancer cells. Biomolecules, 10(3), 447.

Yadav, P. K., Vitvitsky, V., Carballal, S., Seravalli, J., & Banerjee, R. (2020). Thioredoxin regulates human mercaptopyruvate sulfurtransferase at physiologically-relevant concentrations. Journal of Biological Chemistry, 295(19), 6299-6311.

Augsburger, F., & Szabo, C. (2020). Potential role of the 3-mercaptopyruvate sulfurtransferase (3-MST)—hydrogen sulfide (H2S) pathway in cancer cells. Pharmacological research, 154, 104083.

Akahoshi, N., Minakawa, T., Miyashita, M., Sugiyama, U., Saito, C., Takemoto, R., ... & Ishii, I. (2020). Increased urinary 3-mercaptolactate excretion and enhanced passive systemic anaphylaxis in mice lacking mercaptopyruvate sulfurtransferase, a model of mercaptolactate-cysteine disulfiduria. International Journal of Molecular Sciences, 21(3), 818.

Hipólito, A., Nunes, S. C., Vicente, J. B., & Serpa, J. (2020). Cysteine aminotransferase (CAT): a pivotal sponsor in metabolic remodeling and an ally of 3-mercaptopyruvate sulfurtransferase (MST) in cancer. Molecules, 25(17), 3984.

Panagaki, T., Randi, E. B., & Szabo, C. (2020). Role of hydrogen sulfide and 3-mercaptopyruvate sulfurtransferase in the regulation of the endoplasmic reticulum stress response in hepatocytes. Biomolecules, 10(12), 1692.

Nagahara, N., Tanaka, M., Tanaka, Y., & Ito, T. (2019). Novel characterization of antioxidant enzyme, 3-mercaptopyruvate sulfurtransferase-knockout mice: overexpression of the evolutionarily-related enzyme rhodanese. Antioxidants, 8(5), 116.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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