Mouse Anti-MRPL12 Recombinant Antibody (3B12-1A3) (CBMAB-A5583-LY)

Name: Mouse Anti-MRPL12 Recombinant Antibody (3B12-1A3)
SKU: CBMAB-A5583-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3B12-1A3

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

mitochondrial ribosomal protein L12

Introduction

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Entrez Gene ID

6182

UniProt ID

P52815

Alternative Names

5c5-2; FLJ60124; L12mt; MGC8610; MRP-L31/34; MRPL7; MRPL7/L12; RPML12

Function

As a component of the mitochondrial large ribosomal subunit, it plays a role in mitochondrial translation (PubMed:23603806).

Associates with mitochondrial RNA polymerase to activate transcription.

Biological Process

Mitochondrial transcription Source: HGNC-UCL
Mitochondrial translation Source: ComplexPortal
Positive regulation of transcription, DNA-templated Source: HGNC-UCL

Cellular Location

Mitochondrion

Involvement in disease

Combined oxidative phosphorylation deficiency 45 (COXPD45):
An autosomal recessive mitochondrial disorder with onset in utero and characterized by poor overall growth, failure to thrive, global developmental delay, poor or absent speech, seizures, hypotonia, loss of walking, acute progressive neurologic deterioration, brain lesions, and facial dysmorphism. Laboratory studies show increased serum lactate and decreased mitochondrial respiratory chain enzyme activity in patient tissues.

More Infomation

References

Hu, Y., Liu, Y., Ma, C., & Ai, K. (2023). MRPL12 Acts as A Novel Prognostic Biomarker Involved in Immune Cell Infiltration and Tumor Progression of Lung Adenocarcinoma. International Journal of Molecular Sciences, 24(3), 2762.

Ji, X., Yang, X., Gu, X., Chu, L., Sun, S., Sun, J., ... & Wan, Q. (2023). CUL3 induces mitochondrial dysfunction via MRPL12 ubiquitination in renal tubular epithelial cells. The FEBS Journal, 290(22), 5340-5352.

Sun, Y., Liu, Y., Wang, P., Chang, L., & Huang, J. (2023). The m6A Reader YTHDC2 Suppresses Lung Adenocarcinoma Tumorigenesis by Destabilizing MRPL12. Molecular Biotechnology, 1-11.

Ji, X., Chu, L., Su, D., Sun, J., Song, P., Sun, S., ... & Wan, Q. (2023). MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells. Iscience, 26(5).

Gu, X., Liu, Y., Wang, N., Zhen, J., Zhang, B., Hou, S., ... & Feng, H. (2021). Transcription of MRPL12 regulated by Nrf2 contributes to the mitochondrial dysfunction in diabetic kidney disease. Free Radical Biology and Medicine, 164, 329-340.

Yang, Y., Li, C., Gu, X., Zhen, J., Zhu, S., Lv, T., ... & Liu, Y. (2021). ING2 controls mitochondrial respiration via modulating MRPL12 ubiquitination in renal tubular epithelial cells. Frontiers in cell and developmental biology, 9, 700195.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-MRPL12 (AA 1-199) Recombinant Antibody (CBFYM-0512)

Mouse Anti-MRPL12 (AA 1-199) Recombinant Antibody (CBFYM-2553)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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