Mouse Anti-MSRB1 Recombinant Antibody (8B2) (CBMAB-A7986-LY)

Name: Mouse Anti-MSRB1 Recombinant Antibody (8B2)
SKU: CBMAB-A7986-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8B2

Application

WB, ELISA

Immunogen

SEPX1 (NP_057416, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Methionine Sulfoxide Reductase B1

Introduction

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase B (MsrB) family, and it is expressed in a variety of adult and fetal tissues. [provided by RefSeq]

Entrez Gene ID

51734

UniProt ID

Q9NZV6

Alternative Names

HSPC270; MGC3344; MSRB1; SELR; SELX

Function

Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.

Biological Process

Actin filament polymerization Source: UniProtKB
Innate immune response Source: UniProtKB
Protein repair Source: HGNC-UCL

Cellular Location

Nucleus
Cytoskeleton
Cytoplasm

PTM

Truncated MSRB1/SEPX1 proteins produced by failed UGA/Sec decoding are ubiquitinated by some Cul2-RING E3 ubiquitin-protein ligase complexes (containing either PRAME, PRAMF6, PRAMF9 or FEM1C as substrate-recognition component).

Tarrago, L., Kaya, A., Kim, H. Y., Manta, B., Lee, B. C., & Gladyshev, V. N. (2022). The selenoprotein methionine sulfoxide reductase B1 (MSRB1). Free Radical Biology and Medicine.

Yoo, H. J., Choi, D. W., Roh, Y. J., Lee, Y. M., Lim, J. H., Eo, S., ... & Kim, J. H. (2022). MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation. Cell Reports, 41(6).

Chen, X. Y., Yang, S. Y., Ruan, X. J., Ding, H. Y., Wang, N. X., Liu, F., ... & Li, Y. (2021). MsrB1 Promotes Proliferation and Invasion of Colorectal Cancer Cells via GSK-3β/β-catenin Signaling Axis. Cell transplantation, 30, 09636897211053203.

Shi, T., Song, J., You, G., Yang, Y., Liu, Q., & Li, N. (2021). The function of selenium in central nervous system: lessons from MsrB1 knockout mouse models. Molecules, 26(5), 1372.

Wojciechowska, N., Bagniewska-Zadworna, A., Minicka, J., Michalak, K. M., & Kalemba, E. M. (2021). Localization and dynamics of the methionine sulfoxide reductases msrb1 and msrb2 in beech seeds. International Journal of Molecular Sciences, 22(1), 402.

Lee, H. J., Park, J. S., Yoo, H. J., Lee, H. M., Lee, B. C., & Kim, J. H. (2020). The selenoprotein MsrB1 instructs dendritic cells to induce T-helper 1 immune responses. Antioxidants, 9(10), 1021.

Stolarska, E., Bilska, K., Wojciechowska, N., Bagniewska-Zadworna, A., Rey, P., & Kalemba, E. M. (2020). Integration of MsrB1 and MsrB2 in the redox network during the development of orthodox and recalcitrant Acer seeds. Antioxidants, 9(12), 1250.

Shi, T., Yang, Y., Zhang, Z., Zhang, L., Song, J., Ping, Y., ... & Li, N. (2019). Loss of MsrB1 perturbs spatial learning and long-term potentiation/long-term depression in mice. Neurobiology of Learning and Memory, 166, 107104.

Jia, Y., Dai, J., Zhang, L., & Xia, H. (2019). Effect of exogenous zinc on MsrB1 expression and protein oxidation in human lens epithelial cells. Biological Trace Element Research, 190, 60-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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