Mouse Anti-MUC16 Recombinant Antibody (CBWJC-4217) (CBMAB-C5565WJ)

Name: Mouse Anti-MUC16 Recombinant Antibody (CBWJC-4217)
SKU: CBMAB-C5565WJ
Rating: 5 (1 reviews)

See all MUC16 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-4217

Application

ICC, IF, IHC-P

Immunogen

CA 125 antigen derived from ascites fluid

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.09% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mucin 16, Cell Surface Associated

Introduction

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes. [provided by RefSeq, May 2017]

Entrez Gene ID

94025

UniProt ID

Q8WXI7

Alternative Names

Mucin 16, Cell Surface Associated; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125; CA125; CA125 Ovarian Cancer Antigen; Mucin-16; CA-125; MUC-16;

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Biological Process

Cell adhesion Source: UniProtKB

Cellular Location

Extracellular space
Plasma membrane
Cell membrane
Note: May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Topology

Extracellular: 1-14451
Helical: 14452-14472
Cytoplasmic: 14473-14507

PTM

Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

Giamougiannis, P., Martin-Hirsch, P. L., & Martin, F. L. (2021). The evolving role of MUC16 (CA125) in the transformation of ovarian cells and the progression of neoplasia. Carcinogenesis, 42(3), 327-343.

Liu, J., Li, L., Luo, N., Liu, Q., Liu, L., Chen, D., ... & Xi, X. (2021). Inflammatory signals induce MUC16 expression in ovarian cancer cells via NF‑κB activation. Experimental and Therapeutic Medicine, 21(2), 1-1.

Crawford, A., Haber, L., Kelly, M. P., Vazzana, K., Canova, L., Ram, P., ... & Kirshner, J. R. (2019). A Mucin 16 bispecific T cell–engaging antibody for the treatment of ovarian cancer. Science translational medicine, 11(497), eaau7534.

Chen, X., Li, X., Wang, X., Zhu, Q., Wu, X., & Wang, X. (2019). MUC16 impacts tumor proliferation and migration through cytoplasmic translocation of P120-catenin in epithelial ovarian cancer cells: an original research. BMC cancer, 19(1), 1-12.

Felder, M., Kapur, A., Rakhmilevich, A. L., Qu, X., Sondel, P. M., Gillies, S. D., ... & Patankar, M. S. (2019). MUC16 suppresses human and murine innate immune responses. Gynecologic oncology, 152(3), 618-628.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MUC16 Recombinant Antibody (CBWJC-4217)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-MUC16 Monoclonal Antibody (CBFYR0500) (CAT#: CBMAB-R0500-FY)

Mouse Anti-MUC16 Recombinant Antibody (OC125) (CAT#: CBMAB-C5564-CQ)

Mouse Anti-MUC16 Recombinant Antibody (CBYY-C2677) (CAT#: CBMAB-C4118-YY)

Mouse Anti-MUC16 Monoclonal Antibody (CBFYR0443) (CAT#: CBMAB-R0443-FY)

Mouse Anti-MUC16 Recombinant Antibody (CBWJC-4214) (CAT#: CBMAB-C5562WJ)

Mouse Anti-MUC16 Recombinant Antibody (CBFYM-0348) (CAT#: CBMAB-M0422-FY)

Mouse Anti-MUC16 Monoclonal Antibody (CBFYR0496) (CAT#: CBMAB-R0496-FY)

Mouse Anti-MUC16 Monoclonal Antibody (CBFYR0439) (CAT#: CBMAB-R0439-FY)

Mouse Anti-MUC16 Recombinant Antibody (X325) (CAT#: CBMAB-C12100-LY)

Mouse Anti-MUC16 Recombinant Antibody (15) (CAT#: CBMAB-C3206-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry