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Mouse Anti-N6AMT1 Recombinant Antibody (CBFYH-2666) (CBMAB-H3714-FY)

This product is mouse antibody that recognizes N6AMT1. The antibody CBFYH-2666 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all N6AMT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-2666
Application
WB, IP, ELISA

Basic Information

Specificity
Human
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-6 Adenine-Specific DNA Methyltransferase 1
Introduction
This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.
Entrez Gene ID
29104
UniProt ID
Q9Y5N5
Alternative Names
N-6 Adenine-Specific DNA Methyltransferase 1; N-6 Adenine-Specific DNA Methyltransferase 1 (Putative); HemK Methyltransferase Family Member 2; M.HsaHemK2P; C21orf127; HEMK2; N(6)-Adenine-Specific DNA Methyltransferase 1
Function
Methyltransferase that can methylate proteins and, to a lower extent, arsenic (PubMed:18539146, PubMed:21193388, PubMed:30017583, PubMed:31636962, PubMed:31061526).

Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators (PubMed:31061526).

Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif (PubMed:18539146, PubMed:31632689, PubMed:31636962).

Methylates ETF1 on 'Gln-185'; ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated (PubMed:18539146, PubMed:20606008, PubMed:31636962, PubMed:31061526).

May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid (PubMed:21193388, PubMed:25997655).

It however only plays a limited role in arsenic metabolism compared with AS3MT (PubMed:25997655).
Biological Process
Arsonoacetate metabolic process Source: UniProtKB
Chromatin organization Source: UniProtKB-KW
DNA methylation on adenine Source: UniProtKB
Histone lysine methylation Source: UniProtKB
Methylation Source: UniProtKB
Peptidyl-glutamine methylation Source: UniProtKB
Positive regulation of cell growth Source: MGI
Toxin metabolic process Source: UniProtKB
Cellular Location
Nucleus
PTM
Isoform 1:
Ubiquitinated, leading to its degradation by the proteasome.
Isoform 2:
Ubiquitinated, leading to its degradation by the proteasome.

Wang, M., Zhu, J., Ye, Y., Li, P., Sun, W., & Zhang, M. (2023). N6AMT1 is a novel potential diagnostic, prognostic and immunotherapy response biomarker in pan-cancer. Aging (Albany NY), 15(13), 6526.

Liang, X., Guo, G., Wang, Y., Wang, M., Chen, X., Zhang, J., ... & Zhang, Q. (2023). Arsenic metabolism, N6AMT1 and AS3MT single nucleotide polymorphisms, and their interaction on gestational diabetes mellitus in Chinese pregnant women. Environmental Research, 221, 115331.

Chen, J., Zhuang, Y., Wang, P., Ning, J., Liu, W., Huang, Y., ... & Zhang, D. (2022). Reducing N6AMT1-mediated 6mA DNA modification promotes breast tumor progression via transcriptional repressing cell cycle inhibitors. Cell Death & Disease, 13(3), 216.

Zhou, H. M., Xu, H. J., Sun, R. H., Zhang, M., Li, X. T., Zhao, Y. X., ... & Pan, Z. (2022). DNA N6-methyladenine methylase N6AMT1 controls neuropathic pain through epigenetically modifying Kcnj16 in dorsal horn neurons. Pain, 10-1097.

Woodcock, C. B., Yu, D., Zhang, X., & Cheng, X. (2019). Human HemK2/KMT9/N6AMT1 is an active protein methyltransferase, but does not act on DNA in vitro, in the presence of Trm112. Cell discovery, 5(1), 50.

Leetsi, L., Õunap, K., Abroi, A., & Kurg, R. (2019). The common partner of several methyltransferases TRMT112 regulates the expression of N6AMT1 isoforms in mammalian cells. Biomolecules, 9(9), 422.

Li, W., Shi, Y., Zhang, T., Ye, J., & Ding, J. (2019). Structural insight into human N6amt1–Trm112 complex functioning as a protein methyltransferase. Cell Discovery, 5(1), 51.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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