Mouse Anti-NAIP5 Recombinant Antibody (CBWJN-0713) (CBMAB-N1086-WJ)

This product is a Mouse antibody that recognizes NAIP5. The antibody CBWJN-0713 can be used for immunoassay techniques such as: WB.
See all NAIP5 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse

Clone

CBWJN-0713

Antibody Isotype

IgG2b, κ

Application

Basic Information

Specificity

Mouse

Antibody Isotype

IgG2b, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NLR family, apoptosis inhibitory protein 5

Introduction

NAIP5 is a protein coding gene.

Entrez Gene ID

17951

UniProt ID

Q9R016

Alternative Names

Lgn1; Birc1e; Naip-rs3

Function

Sensor component of the NLRC4 inflammasome that specifically recognizes and binds flagellin from pathogenic bacteria such as Legionella or Salmonella (PubMed:12526741, PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).

Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).

The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri (PubMed:21874021, PubMed:21918512, PubMed:29146805, PubMed:29182158).

May contribute to prevent motor-neuron apoptosis induced by a variety of signals (By similarity).

Biological Process

Defense response to bacterium Source: UniProtKB
Defense response to Gram-negative bacterium Source: UniProtKB
Detection of bacterium Source: UniProtKB
Entry of bacterium into host cell Source: MGI
Inflammatory response Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
Negative regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
Positive regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of JNK cascade Source: MGI
Pyroptosis Source: UniProtKB
Regulation of apoptotic process Source: MGI

Cellular Location

IPAF inflammasome complex

References

Paidimuddala, B., Cao, J., & Zhang, L. (2023). Structural basis for flagellin-induced NAIP5 activation. Science Advances, 9(49), eadi8539.

Paidimuddala, B., Cao, J., Nash, G., Xie, Q., Wu, H., & Zhang, L. (2023). Mechanism of NAIP—NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5. Nature Structural & Molecular Biology, 30(2), 159-166.

Zhang, L., Paidimuddala, B., & Cao, J. (2023). Structural basis for flagellin induced NAIP5 activation. bioRxiv, 2023-06.

Wu, X., & Han, J. (2023). Protocol for reconstitution of oligomeric assembly of NAIP5-NLRC4 inflammasome in vitro. STAR protocols, 4(4), 102581.

Paidimuddala, B., Cao, J., Nash, G., Xie, Q., Wu, H., & Zhang, L. (2023). Author Correction: Mechanism of NAIP—NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5 (Nature Structural & Molecular Biology,(2023), 30, 2,(159-166), 10.1038/s41594-022-00889-2). Nature Structural and Molecular Biology, 30(2), 233.

Gonçalves, A. V., Margolis, S. R., Quirino, G. F., Mascarenhas, D. P., Rauch, I., Nichols, R. D., ... & Zamboni, D. S. (2019). Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila. PLoS pathogens, 15(6), e1007886.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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