Mouse Anti-NANOG Recombinant Antibody (NNG-811) (CBMAB-N1118-WJ)

Name: Mouse Anti-NANOG Recombinant Antibody (NNG-811)
SKU: CBMAB-N1118-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

NNG-811

Application

WB, IP, ELISA, IHC-P, ICC

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

2.6 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nanog Homeobox

Introduction

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Entrez Gene ID

79923

UniProt ID

Q9H9S0

Alternative Names

Nanog Homeobox; Homeobox Transcription Factor Nanog; HNanog;

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768).

Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Biological Process

Cell differentiation Source: HGNC-UCL
Endodermal cell fate specification Source: MGI
Positive regulation of stem cell proliferation Source: HGNC-UCL
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of cell differentiation Source: HGNC-UCL
Regulation of gene expression Source: UniProtKB
Regulation of transcription, DNA-templated Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Somatic stem cell population maintenance Source: UniProtKB
Stem cell population maintenance Source: BHF-UCL

Cellular Location

Nucleus

Kuznetsova, K., Chabot, N. M., Ugolini, M., Wu, E., Lalit, M., Oda, H., ... & Vastenhouw, N. L. (2023). Nanog organizes transcription bodies. Current Biology, 33(1), 164-173.

Vasefifar, P., Motafakkerazad, R., Maleki, L. A., Najafi, S., Ghrobaninezhad, F., Najafzadeh, B., ... & Baradaran, B. (2022). Nanog, as a key cancer stem cell marker in tumor progression. Gene, 827, 146448.

Yoon, C., Lu, J., Yi, B. C., Chang, K. K., Simon, M. C., Ryeom, S., & Yoon, S. S. (2021). PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas. Oncogenesis, 10(1), 12.

Najafzadeh, B., Asadzadeh, Z., Motafakker Azad, R., Mokhtarzadeh, A., Baghbanzadeh, A., Alemohammad, H., ... & Baradaran, B. (2021). The oncogenic potential of NANOG: An important cancer induction mediator. Journal of cellular physiology, 236(4), 2443-2458.

Springer, C., Zakhartchenko, V., Wolf, E., & Simmet, K. (2021). Hypoblast formation in bovine embryos does not depend on NANOG. Cells, 10(9), 2232.

Fatma, H., Siddique, H. R., & Maurya, S. K. (2021). The multiple faces of NANOG in cancer: a therapeutic target to chemosensitize therapy-resistant cancers. Epigenomics, 13(23), 1885-1900.

Grubelnik, G., Boštjančič, E., Pavlič, A., Kos, M., & Zidar, N. (2020). NANOG expression in human development and cancerogenesis. Experimental Biology and Medicine, 245(5), 456-464.

Grubelnik, G., Boštjančič, E., Grošelj, A., & Zidar, N. (2020). Expression of NANOG and its regulation in oral squamous cell carcinoma. BioMed Research International, 2020.

Mahalaxmi, I., Devi, S. M., Kaavya, J., Arul, N., Balachandar, V., & Santhy, K. S. (2019). New insight into NANOG: A novel therapeutic target for ovarian cancer (OC). European journal of pharmacology, 852, 51-57.

Heurtier, V., Owens, N., Gonzalez, I., Mueller, F., Proux, C., Mornico, D., ... & Navarro, P. (2019). The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nature communications, 10(1), 1109.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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