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  • Mouse Anti-NANOS2 Recombinant Antibody (CBWJN-1166)

Mouse Anti-NANOS2 Recombinant Antibody (CBWJN-1166) (CBMAB-N1125-WJ)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBWJN-1166
Application
WB
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Nanos C2HC-Type Zinc Finger 2
Introduction
Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
Entrez Gene ID
339345
UniProt ID
P60321
Alternative Names
Nanos C2HC-Type Zinc Finger 2; NOS-2; NOS2; Nanos Homolog 2 (Drosophila); Nanos Homolog 2; ZC2HC12B;
Function
Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
Biological Process
Germ-line stem cell population maintenance Source: UniProtKB
mRNA catabolic process Source: UniProtKB
Negative regulation of meiotic nuclear division Source: UniProtKB
Negative regulation of translation Source: BHF-UCL
Oogenesis Source: GO_Central
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: BHF-UCL
Spermatogenesis Source: UniProtKB
Cellular Location
Cytoplasm
Other locations
P-body
perinuclear region
Note: Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.

Noughabi, M. K., Matin, M. M., Farshchian, M., & Bahrami, A. R. (2023). Immunomodulatory Properties of Mouse Mesenchymal Stromal/Stem Cells Upon Ectopic Expression of Immunoregulator Nanos2. Stem Cell Reviews and Reports, 19(3), 734-753.

Hirano, T., Wright, D., Suzuki, A., & Saga, Y. (2022). A cooperative mechanism of target RNA selection via germ-cell-specific RNA-binding proteins NANOS2 and DND1. Cell Reports, 39(11).

Shimada, R., Koike, H., Hirano, T., Kato, Y., & Saga, Y. (2021). NANOS2 suppresses the cell cycle by repressing mTORC1 activators in embryonic male germ cells. Iscience, 24(8).

Wright, D., Kiso, M., & Saga, Y. (2021). Genetic and structural analysis of the in vivo functional redundancy between murine NANOS2 and NANOS3. Development, 148(1), dev191916.

Codino, A., Turowski, T., Van De Lagemaat, L. N., Ivanova, I., Tavosanis, A., Much, C., ... & O'carroll, D. (2021). NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells. Iscience, 24(7).

Ciccarelli, M., Giassetti, M. I., Miao, D., Oatley, M. J., Robbins, C., Lopez-Biladeau, B., ... & Oatley, J. M. (2020). Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2 knockout males. Proceedings of the National Academy of Sciences, 117(39), 24195-24204.

Imai, A., Hagiwara, Y., Niimi, Y., Tokumoto, T., Saga, Y., & Suzuki, A. (2020). Mouse dead end1 acts with Nanos2 and Nanos3 to regulate testicular teratoma incidence. PLoS One, 15(4), e0232047.

Zhang, J., Han, X., Wang, J., Liu, B. Z., Wei, J. L., Zhang, W. J., ... & Chang, Y. Q. (2019). Molecular cloning and sexually dimorphic expression analysis of nanos2 in the sea urchin, Mesocentrotus nudus. International Journal of Molecular Sciences, 20(11), 2705.

Oulhen, N., Swartz, S. Z., Wang, L., Wikramanayake, A., & Wessel, G. M. (2019). Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways. Developmental biology, 452(1), 34-42.

Shimada, R., Kiso, M., & Saga, Y. (2019). ES-mediated chimera analysis revealed requirement of DDX6 for NANOS2 localization and function in mouse germ cells. Scientific Reports, 9(1), 515.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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